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An electrophysiological study of endothelium-dependent vascular motility

Research Project

Project/Area Number 63560287
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionGIFU UNIVERSITY

Principal Investigator

TAKEWAKI Tadashi  Gifu University, Agriculture, Associate professor, 農学部, 助教授 (00021717)

Co-Investigator(Kenkyū-buntansha) OHASHI Hidenori  Gifu University, Agriculture, Professor, 農学部, 教授 (40001531)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsEndothelium / Voltage-dependent / Ion Channel / Aorta / Time-dependent / パッククランプ / 電位依存性 / 時間依存性 / 血管平滑筋 / アセチルコリン / 膜電位 / 膜電流
Research Abstract

The purpose of this experiment was to investigate whether channels are present in endothelial cells that may be involved in resting potential or the output of endothelium-derived releasing factor(s).
(1) Three types of single channel currents were elicited at membrane potentials more positive than the resting potential.
(2) The reversal potentials of the three types of single channel currents were at 0mV, which remained unaltered after substitution of Na or Ca ion for K ion in the bathing solution. Thus, these three types of channels are non- selective cation channels.
(3) The slope conductances were calculated to be 100,50 and 20 pS.
(4) The open probability of the middle type of channel increased as the Ca ion concentration in the bath solution was increased. The activity of the other channel was also dependent on the Ca ion concentration of the bath solution.
(5) The present results suggest that the non-selective cation channels of the guinea-pig aorta may be kept close at the resting potential and activated after depolarization of the membrane potential, and that the channel may be inactivated voltage-dependently and time-dependently.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] T.Takewaki,et al.: "Possible role of prostaplandins in requlating the non-cholinergic,non-adrenergic excitatory neuronal control of the rabbit urinery blalpes." Res.Bull.Fac.Agr.Gifu Univ. 53. 356-362 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Takagi et al.: "Development of the intramural innervation in the gizzard of the chicken" Jpn.J.Pharmacol. 49. 202 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Takewaki,et al.: "Inhibitory effect of hemicholinium-3 on the acetylcholine out put and the perstatlic roflax in the isolated quine-pig intestine" Res.Bull.Fac.Agr.Gifu Univ. 54. 217-224 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] F.Melcata et al.: "Regulation of potassium channel in electrical stability of the smooth muscle all membrare of the guirem-pig aorta" Pflugers Arch.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Takewaki et al.: "Development of the intramural cholinegic and run-chelinegic innerration in the gizzard of the chicken" Brit.J.Pharmacol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Takewaki et al.: "Single voltage-dependent non-solective cation channel in endotheliel alls of the quinea-pig aorta." Pfliigers Arch.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Takewaki et al.: "Possible role of prostaglandins in regulating the non-cholinergic, non-adrenergic excitatory neuronal control of the rabbit urinary bladder." Res. Bull. Fac. Agr. Gifu Univ. 53: 356-362, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Takagi et al.: "Development of the intramural innervation in the gizzard of the chicken." Jpn. J. Pharmacol. 49: 202, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Takewaki et al.: "Inhibitory effect of hemicholinium-3 on the acetylcholine output and the peristaltic reflex in the isolated guinea-pig intestine." Res. Bull. Fac. Agr. Gifu Univ. 54: 217-224, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] F. Mekata et al.: "Regulation of potassium channel in electrical stability of the smooth muscle cell membrane of the guinea- pig aorta." Pflugers Arch.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Takewaki et al.: "Development of the intramural cholinergic and non-cholinergic innervation in the gizzard of the chicken." Brit. J. Pharmacol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Takewaki et al.: "Single voltage-dependent non-selective cation channel in endothelial cells of the guinea-pig aorta." Pflugers Arch.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Takewaki & S.Utumi: "Inhibitury effect of hemichilinium-3 on the acetylcholine Output and the perijtaltic neflex in the isolated guihea-pig intestine" Res.Bull.Fac.Gifu Univ.54. 217-224 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] F.Mekata & T.Takewaki: "Regutation of potassium channel in electrical stability of the smooth muscle cell membrane of the guinela-pig aorts" Pflugers Arch.

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Takewaki,N.Takagi & H.Ohashi: "Developmect of the intramunal mnervation in the gizzard of the chken" Brit.J.Pharmacal.

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Takewaki,N.Masuda & H.Ohashi: "Single vollye-depondit non-selective cation channels in and the lial cells of gumca-pyuortr" Pflugers Arch.

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Takagi,T.Takewaki & H.Ohashi: "Development of the intramunal innervation in the gizzard of the chicken" Jpn.J.Pharmacal. 49. 202 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Takewaki: "Poesible role of prostagladirs in regulating the non-cholmengi、non-adrenergie excitatory reucenel control of rabbit urinnary bladder." Res.Bull.Fac.Agr.Gifu Univ.53. 356-362 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Takewaki,;M.Asahi;H.Ohashi: Research Bulletin of the faculty of Agriculture Gifu Unversity. 53. 353-362 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] N.Takagi;T.Takewaki;H.Ohashi: japanese Journal of pharmacology. 49. 202 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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