| Project/Area Number |
63570072
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurophysiology and muscle physiology
|
|---|
| Research Institution | UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, SCHOOL OF MEDICINE |
|---|
Principal Investigator |
INENAGA Kiyotoshi UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (90131903)
|
|---|
| Project Period (FY) |
1988 – 1989
|
| Project Status |
Completed (Fiscal Year 1989)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | HYPOTHALAMUS / PATCHCLAMP METHOD / OSMOSENSITIVITY / SLICE / OPIOID / パッチ・クランプ法 / 単離した視床下部神経細胞 / 視索上核 / 第3脳室側壁部 / 瞬時外液交換法 / ナトリウム電流 |
|---|
| Research Abstract |
1. To test osmosensitivity of hypothalamic neurons, the patch clamp method and the concentration clamp were used in the present study. Whole cell recordings were obtained from neurons in the regions of the anteroventral third ventricle (AV3V) which were isolated enzymatically and mechanically from 7 to 15-day-old rats. We analyzed chance of voltage-dependent sodium current with osmotic challenge. The results demonstrated the view that osmotic stimulation acts on neurons located in the AV3V region by modifying their membrane permeability for sodium ions with a slow kinetics.
2. To clarify opioid-response of supraoptic neurons in the hypothalamus, slice preparation and extracellular recordings were used. The results suggest that opioids acting preferentially at kappa-receptors inhibit both vasopressin and oxytocin neurons while delta- and mu-receptor agonists inhibit primarily oxytocin neurons.
|
