• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of Adenylate Cyclase Activity by a GTP-Binding Peptida in Rat Liver.

Research Project

Project/Area Number 63570105
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKansai Medical University

Principal Investigator

OMORI Kyoko  Kansai Med. Univ., Dpt. of Pharmacol., Lecturer, 医学部 (90152256)

Co-Investigator(Kenkyū-buntansha) OTANI Hitomi  Kansai Med. Univ., Dpt. of Pharmacol., Assistant, 医学部, 助手 (40140272)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
KeywordsAdenylate Cyclase / beta-Adrenergic Response / Rat Liver / Cytosolic Factor / GTP-Binding / Peptide / アデニレ-トシクラ-ゼ / β-アドレナリン性応答 / アデニレートシクラーゼ
Research Abstract

A cytosolic peptide ・ GTP complex which stimulates iota -isoproterenol- responsive adenylate cyclase activity was identified in the rat liver. The peptide component was purified and characterized with regard to its interaction with GTP in the regulation of adenylate cyclase. The peptide was isolated as a complex form with GTP on a Sephadex G-25 column in 1 mM NaHCO_3, and on the same column in 200 mM ammonium acetate it was purified as a dissociated form with Mr -3,000. The purified peptide alone displayed a markedly decreased stimulatory effect on adenylate cyclase activity, but its reassociated form with GTP clearly enhanced the effect of GTP on the enzyme activity. The isoproterenol competition curve using iota -[^3H]dihydroalprenolol (DHA) as an antagonist ligand shifted to lower affinity by the addition of the peptide reassociated with GTP (16.5-fold shift), while the same concentration of GTP (1 mu M) or the peptide alone had little or no effect (1.5- or 0.9-fold shift, respectively). Furthermore, the peptide enhanced the GTP effect in the response to iota -isoproterenol but not in that to glucagon, prostaglandin E_1 and fluoride. These results suggest that the peptide component of the cytosolic activator potentiates the effect of GTP on the agonist- beta -adrenergic receptor-stimulatory guanine nucleotide-binding regulatory component of adenylate cyclase (G_s) (GDP) ternary complex.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kyoko Omori: "A Cytosolic Peptide Potentiates the GTP Effect on βーAdrenergic Response of Adenylate Cyclase."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiko Uriu: "Two Types of ClathrinーCoated Vesicles Isolated from Rat Brain:Analysis of Biochemical Properties and cellular origin." J.Neurochem.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koichiro Omori: "Expression,Localization,and Function of an NーTerminal Half Fragment of the Rat Na,KーATPase βーSubunit in HeLa Cells." J.Biochem.109. 267-275 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kyoko Omori: "Involvement of a cytosolic peptide・GTP complex in stimulation of hormoneーsensitive adenylate cyclase activity in rat liver." Jpn.J.Pharmacol.49. 66T- (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kyoko Omori: "A Cytosolic Peptide Potentiates the GTP Effect on beta-Adrenergic Response of Adenylate Cyclase." American J. Physiol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiko Uriu: "Two Types of Clathrin-Coated Vesicles Isolated from Rat Brain : Analysis of Biochemical Properties and Cellular Origin." J. Neurochem.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koichiro Omori: "Expression, Localization, and Function of an N-Terminal Half Fragment of the Rat Na, K-ATPase beta-Subunit in HeLa Cells." J. Biochem.109. 267-275 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kyoko Omori: "Involvement of a cytosolic peptide・GTP complex in stimulation of hormone-sensitive adenylate cyclase activity in rat liver." Jpn. J. Pharmacol.49. 66 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kyoko Omori: "A Cytosolic Peptide Potentiates the GTP Effect on βーAdrenergic Response of Adenylate Cyclase."

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshiko Uriu: "Two Types of ClathrinーCoated Vesicles Isolated from Rat Brain:Analysis of Biochemical Properties and Cellular Origin." J.Neurochem.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Koichiro Omori: "Expression,Localization,and Function of an NーTerminal Harf Fragment of the Rat Na,KーATPase βーSubunit in HeLa Cells." J.Biochem.109. 267-275 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshiko Uriu: "A vanadateーsensitive ATPase of clathrinーcoated vesicles from rat brain." Jpn.J.Pharmacol.55. 171 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tohru Yasukura: "Purification of retinal cholinergic neuronーneurotrophic factor derived from cultured hippocampal neurons." Jpn.J.Pharmacol.55. 347 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tohru Yasukura: "Stimulation of the development of cholinergic retinal neurons from the rat fetus in culture by a hippocampus neuron-derived factor." Jpn.J.Pharmacol.52. 223p (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Toshiko Uriu: "Isolation and characterization of two populations of clathrin-coated vesicles in the rat brain." Jpn.J.Pharmacol.52. 224p (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Toshiko Uriu: "Two types of clathrin-coated vesicles in the rat brain:Analysis for cellular origin"

    • Related Report
      1989 Annual Research Report
  • [Publications] Koichiro Omori: "Expression,Localization,and Function of a N-Terminal Half Fragment of the rat Na,K-ATPase β-subunit in Hela Cells."

    • Related Report
      1989 Annual Research Report
  • [Publications] Kyoko Omori: "A novel cytosolic peptide component potentiates the stimulatory effect of GTP on hormone-sensitive adenylate cyclas activity in rat liver."

    • Related Report
      1989 Annual Research Report
  • [Publications] Koichiro Omori.: Progress in Clinical and Biological Research. 268 B. 127-134 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Toshiko Uriu.: Jpn.J.Pharmacol.49. 276P (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kyoko Omori.: Jpn.J.Pharmacol.49. 66P (1988)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi