| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
After induction of experimental autoimmune thyroiditis (EAT) in mice, long-term observation on both the severity of thyroiditis and antibody formation against mouse thyroglobulin (MTg) was carried out over a period of 18 months. Both the severity of thyroiditis and MTg antibody production continued for a long term in EAT-susceptible strains (CBA, C3H). In EAT-resistant mice (BALB/c), thyroiditis remained minimal, if present, and the low antibody response to MTg became negligible. Antibody against sheep red blood cells (SRBC) in serum, also continued for a long term, depending on mouse strains. On the other hand, aging had a prominent effect on the EAT induction in susceptible mice; both MTg antibody response and thyroid infiltration were markedly reduced. These data were well correlated with reduced antibody formation against SRBC. Young and old mice were further compared as donors or recipients in adoptive transfer of concanavalin A-stimulated cells. Lymph node cells from either young or old MTg-immunized mice transferred appreciable thyroiditis to normal young and old recipient mice. However, similarly treated spleen cells from MTg-immunized old mice responded more poorly to in vitro stimulation with mitogens or MTg and transferred thyroiditis to very few normal young or old mice.
In next experiment, cloning of effector T cells against thyroid epithelial cells should be performed. Further, the response of in vivo immune system should be studied after injection of cloned effector T cells.
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