| Project/Area Number |
63570157
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Yamanashi Medical College |
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Principal Investigator |
KOBAYASHI Makio Yamanashi Medical College, Pathology, Asso. Prof., 医学部病理, 助教授 (80060086)
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| Co-Investigator(Kenkyū-buntansha) |
SAWADA Tatsuo Nihon Univ. School of Med., Pathology, Asso. Prof., 医学部病理, 助教授 (90162544)
TASAKA Kachio Yamanashi Medical College, Immunology, Asso. Prof., 医学部免疫, 助教授 (40093265)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | retinoblastoma / extracellular matrix / glutamate decarboxylase / cell differentiation / SDS-PAGE / フィブロネクチン / 細胞芽細胞腫 / 培養細胞 / 糖鎖構造 |
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| Research Abstract |
The histogenesis of retinoblastoma(Rb), the most common introocular tumor of childhood, has been recently attributed to a primitive retinal cell which is capable of differentiation of Y79 cell line into several cell types was investigated. By manipulating the culture medium constitutes and using differentiating agent as dibutyryl cysclic AMP. Electronmicroscopy, immunohistochemistry for detection of glutamate decarboxilase(GAD) and for extracellular matrix(ECM) such as fibronectin and stromal collagen(IV). Treated cells exhibited morphologic characteristics supportive of differentiation toward photoreceptors, increased GAD reactivity, and glycoconjugate expression. It was found that treated cells synthesize ECM in characteristic patterns. No stromal collagen synthesis was observed. These result confirm the concept that human Rb originates from a primitive neuroectodermal multipotential cell and contributes to the possibility of induction of differentiation therapy for Rb.
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