| Project/Area Number |
63570166
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Saga Medical School |
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Principal Investigator |
SUGIHARA Hajime Saga Medical School, Pathology, Prof., 医学部, 教授 (50039509)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Shuji Saga Medical School, Pathology, Assist., 医学部, 助手 (80188755)
YONEMIYSU Nobuhisa Saga Med. Sch. Pathology, Assist., 医学部, 助手 (60145191)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | obese / fat cells / Growth Factor / Proliferation / Differentiation / collagen / Cell Biology / Insulin / コラーゲン |
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| Research Abstract |
The establishment of a culture system of fat cells is a prerequisite for study of the cell biology of fat cells of the obese.Mature white fat cells do not attach themselves to the bottom surface of a culture dish when incubated in medium. Instead, they tend to float on top of the medium because of their high lipid content. We succeeded a new method for culturing mature fat tissue fragments. Fibroblast-like fat cells derived from the fat tissue proliferated extensively. "Adipocytes Growth Factor" was studied with this culture method.
As the result, the conditioned medium of the fat cells, insulin and callagen accelerated the proliferation in the culture condition. Some substance shall be analyzed as the "Adipocytes Growth Factor" from the conditioned medium. The effect of insulin is nonspecific. Collagen is a essential extracellular matrix for the fat cells. The effect of collagen is thought to be reasonable to the proliferation and differentiation of the fat cells.
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