| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
1) Digestion, homogenizing and pressing methods for collecting metacercariae from crabs were compared. As a result, successive use of pressing and homogenizing methods provided the highest efficiency.
2) Pick-up, quadrat and trap methods for sampling crabs were compared in respect of infection levels of the crab population. It was concluded that the quadrat method should be employed to estimate infection parameters, and parameters from crabs larger than a threshold size should be taken to comparewith those in other localities.
3) Lung flukes are widely distributed in the western Shizuoka Prefecture and around Shinshiro-city, Aichi Prefecture, though at a low level. No infected crabs were found in northern area to Shinshiro-city. Infection level of wild mammals were very low.
4) Paragonimus westermani and P. miyazakii coexist in the same crab population at Mikkabi-cho, Shizuoka Prefecture. Although morphology of the ovary of P. westermani resembled that of P. miyazakii in pressed speciment
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s, a protein analysis revealed clear difference between the two. The first intermediate host snail of P. westermani was found on the study site, although snail host for P. miyazakii was not found. This suggests that distribution of host snails could be an important factor for the coexistence of the two species. Sympatric distribution by closely related species with the same ecological niche would be worth investigating further.
5) In experimental infection of dogs and rats, 16% of recovered worms were P. westermani. Those in rats were often found in the plural cavity. Most individuals of different species did not share the same cyst, remaining species independency.
6) To clarify morphological difference in the ovary of the two species, three-dimensional reconstruction technique was employed. In P. westermani, although branching structure was variable, all individuals examined had a common basic structure consisting of 6 lobes. In P. miyazakii the structure was complicated, suggesting a fundamental difference between the two species. Less
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