| Project/Area Number |
63570182
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Kitasato University |
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Principal Investigator |
YANAGISAWA Toshio Kitasato Univ. Sch. of Med. Prof., 医学部, 教授 (20050312)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Takeshi Kitasato Univ. Sch. of Med. Assistant Prof., 医学部, 講師 (30050652)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Spirometra erinacei / Plerocercoid / Cysteine protease / マンソン裂頭条虫 / プレロセルコイド / 孤虫症 / チオールプロテアーゼ |
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| Research Abstract |
Thiol protease was isolated from plerocercoid of Spirometra erinacei (plerocercoid protease, pl-p). Splitting activities of the protease against structural and contractile proteins from host vertebrate skeletal muscle were investigated. the results obtained are as follows.
1) Pl-P may cleave native actin and myosin peptides. G-actin is digested by pl-p more quickly than F-actin. ATPase activity remained intact on the residues produced by myosin digestion.
2) A myofibril prepared from host skeletal muscle, having macromolecular structure may also digested by pl-p in vitro. myosin, a constituent of the thick-filament on, myofibril, is splitted and dissolved without a loss of its ATPase activity.
3) Effects of pl-p on the host actomyosin containing a regulatory system (myosin-B) were examined by changes in superprecipitation as an in vitro model of muscular contraction. Ca^<2+>-sensitivity in the actomyosin system is lost by the addition of pl-p.
4) The effect of pl-p on regulatory proteins, tropomyosin and troponin was examined. Substrates used are cleaved more preferably cleaved by pl-p in the following order: troponin T-subunit<greater than or equal> troponin I-subunit > tropomyosin > troponin C
Results obtained from the present study suggest that pl-p from S. erinacei plerocercoids may be able to degradate host muscular structure.
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