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Studies on glycolipids of Trypanosoma cruzi

Research Project

Project/Area Number 63570184
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionTOKAI UNIVERSITY

Principal Investigator

KANEDA Yoshimasa  Tokai Univ., Sch. of Med., Dep"t of Parasitology, Professor, 医学部・寄生虫学, 教授 (60051471)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsTrypanosoma cruzi / glycolipid / antigenicity / liposome / クルーズ / トリパノソーマ
Research Abstract

The antigenic properties of glycolipids and their importance as immunogens have been well established (Alving, 1977; Marcus and Schwarting, 1976; Hakomori, 1981). Blood group determinants, O antigens in murine thymus and brain tissues, Forsman antigens and receptors for bacterial toxins have been found to be carried by glycolipids which participate in various reaction with numerous biological substances including lectins. Recently, we demonstrated glycolipids in the lipid extracts of the epimastigote forms of T. cruzi cultured in a liver- infusion-tryptose medium using by thin layer chromatography on a silicic acid (Nagakura and Kaneda, 1982). During this project, we continued to characterize the antigenic properties of glycolipid fraction derived from T. cruzi by enzyme-linked immunoassay method (ELISA) and studied on the antigenic differences between several strains of the parasites. We had shown that the fraction was antigenic because specific antibodies were detected in the sera of infected mice (Kaneda et al., 1987). Furthermore, we produced several monoclonal antibodies against T. cruzi. The reactivity of the monoclonal antibodies and the glycolipid fraction was determined by the ELISA test. One of the monoclonal antibodies, which recognized antigenic epitopes on the surface of all stages of the parasite, strongly reacted to the glycolipid fraction. Based on these observations, we suggested that the glycolipid fraction may exist on the surface membrane of T. cruzi. In our laboratory, we are able to grow large number of parasites in culture, assuring a readily available supply of antigen. We plan to study the usefulness of this glycolipid antigen in the diagnosis of Chagas' disease.
In another study, we examined the cytotoxic effects of stearylamine-bearing liposomes against trypomastigotes of T. cruzi. Exposure of the parasites to the liposomes caused degenerative changes. These results represent a promising new strategy for the therapy of Chagas' disease.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 橘裕司、永倉貢一、金田良雅、北潔、他: "モノクロ-ナル抗体によるTrypanosoma cruzi抗原の解析" 寄生虫学雑誌. 36. 113 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 金田良雅、田中朝雄、Than Saw,他: "嫌気性原虫類の増殖に対する植物根より抽出したBerberineの影響" 寄生虫学雑誌. 38. 91-92 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 田中朝雄・金田良雅: "ELISAおよびImmunoflotting法を用いたトリコモナス感染のスクリ-ニング法" 寄生虫学雑誌. 38. 78 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Tachibana, K. Nagakura, Y. Kaneda, K. Kita, M. Kawabata, T. Mimori, Y. Hashiguchi and N. Takasu: "Analysis of Trypanosoma cruzi antigens by using monoclonal antibodies. (V)Serodiagnosis of Chagas' disease by a competition ELISA." Jpn. J. Parasitol. vol.36, 112, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Kaneda, T. Tanaka, Than Saw, F. Yamamura and Y. Kato: "Effects of the berebrine extracted from "Khant Htauk Myit" on the growth of anaerobic protozoa." Jpn. J. Parasitol. vol.38, 91, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Tanaka and Y. Kaneda: "Screening survey of trichomonas infection using ELISA and immunoblotting test." Jpn. J. Parasitol. vol.38, 78, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 橘裕司、永倉貢一、金田良雅、北潔、他: "モノクロ-ナル抗体によるTrypanosoma cruzi抗原の解析" 寄生虫学雑誌. 36. 113 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] 金田良雅、田中朝雄、Than Saw、他: "嫌気性原虫類の増殖に対する植物根より抽出したBerberineの影響" 寄生虫学雑誌. 38. 91-92 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 田中朝雄、金田良雅: "ELISAおよびImmunoblotting法を用いたトリコモナス感染のスクリ-ニング法" 寄生虫学雑誌. 38. 78 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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