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Studies on the Protein Synthesis by Toxoplasma Gondii in Cell Free System.

Research Project

Project/Area Number 63570186
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research Institution国立予防衛生研究所

Principal Investigator

ENDO Takuro  N. I. H., Department of Parasitology, Head of lst Division., 寄生虫部, 室長 (90072959)

Co-Investigator(Kenkyū-buntansha) KAWANAKA Masanori  N. I. H., Department of Parasitology, Head of 3rd Division., 寄生虫部, 室長 (50109964)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsToxoplasma gondii / Tachyzoite / Methionine uptake / External ionic condition / Electrochemical H^+ gradient / Radiolabelling / トキソプラズマ原虫 / アミノ酸の取り込み / メチオニン / pH / アミノ酸吸収 / カリウムイオン / ナトリウムイオン / 細胞内pH / 塩素イオン / 硫酸塩
Research Abstract

The main reason for studying Toxoplasma is to learn more about its behavior in hopes of subsequently discovering a method for controlling or eradicating the disease it causes. Despite intensive study by experimental infection of various animals and tissue cultures, relatively little is known about this parasite's requirements for infection, survival and growth in a host cell. Nutritional studies of this parasite are complicate by its obligate parasitic nature. They are hidden from view of most of their life cycle. The extra-host cellular stage of the parasite, namely tachyzoite, which is easily obtainable in quantity seemed to be inactive or depressed, if any, in its metabolic activity. We examined influences of extracellular ions on several cellular events of this parasite including regulation of internal pH, motility and infectivity. We also studied possible occurrence of incorporation of ^<35>S-methionine by the tachyzoites in cell-free medium with varying external ionic conditions. In the present report, external ionic conditions, mainly K^+ and C1^-, are discussed in terms of electrochemical H^+ gradients across the parasite's membrane for cellular events of the tachyzoite in cell-free medium. A successful application of metabolic radiolabelling of toxoplasma proteins by ^<35>S-methionine let us possible tracer experiments of this parasitic protozoon. This method may also useful for screening anti-toxoplasma agints.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] ENDO,T.,Yagita,K.,Yasuda,T. & Nakamura,T.: "Detection and localization of actin in Toxoplasma gondii" Parasitology Reseanch. 75. 102-106 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuda,T.,Yagita,K.,Nakamura,T.& Endo,T.: "Immunocytochemical localization of actin in Toxoplasma gondii" Parasitology Reseanch. 75. 107-113 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo,T.and Yagita,K: "Toxoplasma gondii : A simple method for titration of infectivity with monolayer cells." Japan.J.Med.Sci.Biol.42. 13-23 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo,T.and Yagita,K.: "Effect of Ext ellulan ions on motility and cell entry in Toxoplasma gondii" J.Protozoology. 37. 133-138 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo,T.,Kawanaka,M.and Yagita,K: "Toxoplasma gondii:amino acid uptake in cellーtree medium" J.Protozoology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo, T. Yagita, K., Yasuda, T. and Nakamura, T.: "Detection and localization of actin in Toxoplasma gondii." Parasitol. Res.75. 102-106 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuda, T., Yagita, K., Nakamura, T. and Endo, T.: "Immunocytochemical localization of actin in Toxoplasma gondii." Parasitol. Res.75. 107-113 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo, T. and Yagita, K.: "Toxoplasma gondii : A simple method for titration of infectivity with monolayer cells." J. J. Med. Sci. Biol. 42. 13-23 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo, T. and Yagita, K.: "Effect of Extracellular ions on motility and cell entry in Toxoplasma gondii." J. Protozool.37. 133-138 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo, T. and Yagita, T.: "Toxoplasma gondii : Amino acid uptake in cell-free medium." J. Protozool.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Endo T.& Yagita K.: "Toxoplasma gondii:A simple method for titration of infectivity with monolayer cells" Japan.J.Med.Sci.Biol.42. 13-23 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Endo T.& Yagita K.: "Effect of extraceclular ions on motility and cell entry in Toxoplasma gondii" J.Protozool. 37. 133-138 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Endo T.& Yagita K.: "Changes in internal pH and in vitro amino and uptake by Toxoplasma gondii" J.Protozool.

    • Related Report
      1990 Annual Research Report
  • [Publications] Endo,T.and Yagita,K: "Toxoplasma gondii:Asimple method for titration of infeetivity with monolayer cells." Japan.J.Med.Sci.Biol.42. 13-23 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Endo,T.and Yagita,K.: "Efect of Extracellular lons on Motility and Cell Entry in Toxoplaema gondii" J.Brotozool. 37. 133-138 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yagita,K and Endo,T.: "Regtriction Enzyme Analysis of Mitochondrial DNA of Acanthamoeba strains in Japan" J.Protozool.

    • Related Report
      1989 Annual Research Report
  • [Publications] Endo,T.;Yagita,K.: Parasitol.Recearch.

    • Related Report
      1988 Annual Research Report
  • [Publications] Endo,T.;Yagita,K.: J.Prrotozool.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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