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CHARACTERIZATION OF THE PLASMID MEDIATED CEPHALOSPORINASE ISOLATED FROM CLINICAL STRAINS IN GRAM-NEGATIVE BACTERIA.

Research Project

Project/Area Number 63570187
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionGUNMA UNIVERSITY

Principal Investigator

INOUE Matsuhisa  SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (10008336)

Co-Investigator(Kenkyū-buntansha) INOUE Matsuhisa  SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR (10008336)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordscephalosporin / cephem resistance / cephalosporinase / beta-lactamse / isoelectric point / cephem plasmid / セフェム耐性 / 基質特異性 / 第3世代セフェム / ペニシリナ-ゼ / プラスミド支配のセファロスポリナーゼ / オキシイミノセファロポリナーゼ / 不和合性 / β-ラクタム剤 / β-ラクタマーゼ
Research Abstract

Infections caused by strains of gram-negative rods resistance to broad spectrum cephalosporins, so called third generation cephems, have been observed recently in Japanese hospitals. During past 5 years study for cepehems resistance, we found cephen resistant plasmids from A.colcoaceticus, E.coli, Proteus sp. and C.freundii. A cephem resistant plasmid from A.calcoaceticus produced typical cephalosporinase. However other cephen resistant plasmids produced broad spectrum cephalosporinases.
The strains produce beta-lactauses isoelectric point close to 9.4 to 9.6. The enzymes efficiently hydolysed cephems including third generation cephems. From the substrate profiles and isoelectric point. these enzymes differ from those of known beta-lactamses including CTX-1.
Molecular cloning of DNA fragments between 3.4 to 3.6 kb EcoR1 and Sph1 restriction endonucleases digested permitted the isolation of structural genes coding for cephlosporinase. No DNA homology was detected between known plasmid DNA and these cephen resistance genes. DNA sequences analysis has been under investigation.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 井上松久,平岡聖樹,岡本了一: "β-ラクタマ-ゼの検査法" 検査と技術. 16. 239-244 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 吉田益史,岡本了一,平岡聖樹,三橋進,田島政三,井上松久: "E.coliの産生するβ-lactamaseの性状について" Chemoherapy. 37. 233-234 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Hikida,M.Yasida,S.Mituhasi,M.Inoue: "Purification and properties of A.cephiospocrinase from A.caloaceticus" J.Antibiotics. 42. 123-126 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Inoue,M.Yoshida,A.Fuse,M.Tajima: "Purification and properties of plasmid mediated cephalosporinase isolated from E.coli and C.freundii." Antimicrob.Agents Chemother.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Inoue and R.Okamoto: "DNA homology of cephem resistant genes between gram-negative bacterial chromosome and plasmid which coded cephalosporinase." Antimicrob.Agents Chemother.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Inoue,R.Okamoto,K.Matsuda: "Characterization of cephalosporinase which mediated by on plasmid" J.Antimicrob.Chemother.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yosida, M., M.Hikida, M.Mitsuhashi, M.Inoue.: "Properties of plasmid mediated cephalosporinase isolated from A.calcoaceticus." Chemotherapy. 36:344, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Inoue, M., S.Hiraoka, R.Okamoto.: "Method for beta-lactamse detection." Modern Medical Laboratory 16:239-244, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshida, M., R.Okamoto, S.Hiraoka, S.Mitsuhashi, M.Tajima, M.Inoue.: "Characterization of plasmid mediated cephalosporinase isolated from E.coli." Chemotherapy 37:233-234, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hikida, M., M.Yosida, S.Mitsuhashi, M.Inoue.: "Purification and properties of a cephalosporinase from A.calcoaceticus." J.Antibiotics 42:123-126, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 井上松久,平岡聖樹,岡本了一: "β-ラクタマ-ゼの検査法" 検査と技術. 16. 239-244 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉田益史,岡本了一,平岡聖樹,三橋進,田島政二,井上松久: "E.coliの産生するβ-lactamaseの性状について" Chemoherapy. 37. 233-234 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Hikida,M.Yasida,S.Mituhasi,M.Inoue: "Purification and properties of A.cephiospocrinase from A.caloaceticus" J.Antibiotics. 42. 123-126 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Inoue,M.Yoshida,A.Fuse,M.Tajima: "Purification and properties of plasmid mediated cephalosporinase isolated from E.coli and C.freundii" Antimicrob.Agents Chemother.

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Inoue,R.Okamoto: "DNA homology of cephem resistant genes between gram-negative bacterial chromosome and plasmid which coded cephalosporinase" Antimicrob Agents Chemother.

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Inoue,R.Okamoto,K.Mstsuda: "Characterizatin of cephalosporinase which medeated by on plasmid" J.Antimicrob,Cheother.

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Hikida 他: J.Antibiotics. 42. 123-126 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Inoue 他: Antimicrobial Agents Chemotherapy.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Inoue 他: Antimicrobial Agents Chemotherapy.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Yoshida 他: Antimicrobial Agents Chemotherapy.

    • Related Report
      1988 Annual Research Report
  • [Publications] 井上松久: "Perspective into Resistance to Antibiotics" 万有製薬株式会社, 33-41 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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