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Analysis of immune bactericidal reaction by human complement component C9-deficient serum

Research Project

Project/Area Number 63570192
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionOsaka University

Principal Investigator

KYONGSU Hong  Osaka University. Bacteriology. Research Associate, 医学部, 助手 (30158894)

Co-Investigator(Kenkyū-buntansha) KINOSHITA Taroh  Osaka University. Bacteriology. Assistant Professor, 医学部, 講師 (10153165)
INOUE Kozo  Osaka University. Bacteriology. Professor, 医学部, 教授 (10028300)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBactericidal activity / C9-deficient serum / Escherichia coli / 免疫殺菌反応 / 膜傷害
Research Abstract

Escherichia coli B/SM, 1-1, was killed dose-dependently by human hereditarily C9-deficient serum(C9DHS) that was shown to contain no C9 antigen by ELISA (Takata et al. J. Immunol. Method. 117: 107, 1989). Bactericidal activity of C9DHS was inhibited by rabbit anti-C5 antibody but not by murine monoclonal anti-C9 antibody. The anti-C9 antibody diminished bactericidal activity of normal human serum(NHS) to the level by C9DHS. Sheep anti-human lysozyme antibody did not affect the bactericidal activity of C9DHS or NHS even when added in an amount more than twice that enough to block the serum lysozyme activity on Micrococcus luteus. The surviving E. coli which had been treated with C9DHS was killed by C9 but not lysozyme, transferrin or both.
other laboratory-kept strains of E. coli ( K12, W3110,C600, NIHJ ) were also killed by C9DHS. However, pathogenic strains isolated from patients of traveler's diarrhea were all resistant to both C9DHS and NHS, at least, at a serum concentration tested. These results show clearly that C9DHS can kill rough strains of E. coli without participation of lysozyme.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] T.Kinoshita: "Monoclonal antibodies to mouse complement receptor type1(CR1):their use in addistribution study showing that mouse erythrocytes and platelets are CR1-negative." J.Immunol.140. 3066-3072 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Kinoshita: "C5 convertase of the alternative complement pathway:covalent linkage between two C3bmolecules within the trimolecular complex enzyme." J.Immunol.141. 3895-3901 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hong: "Inhibitions of the alternative C3 convertase and classical C5 convertase of complement by group A streptococcal M protein." Infec.Immum.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] P.Patcharin.: "Bactericidal activity of C9-deficient human serum."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 洪卿秀(Kyongsu Hong): "H因子(factor H)(β1Hクロブリン)(β1H)" 日本臨床, 6 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 洪卿秀(Kyongsu Hong): "臨床免疫(V補体H因子)" 日本臨床,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Kinoshita: "Monoclonal antibodies to mouse complement receptor typel(CR1): their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative." J. Immunol.140. 3066-3072 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Kinoshita: "C5 convertase of the alternative complement pathway: covalent linkage between C3b molrcules within the trimolecular complex enzyme." J. Immunol. 141.3895-3901.1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Hong.: "Inhibitions of the alternative C3 convertase and classical C5 convertase of complement by group A streptococcal M protein." Infect. Immun.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] P. Patcharin: "Bactericidal activity of C9-deficient human serum."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kyongsu Hong: "Inhibitions of the alternative C3 convertase and classical C5 convertase of complement by group A streptococcal M protein." Infect.Immun.

    • Related Report
      1989 Annual Research Report
  • [Publications] P.Patcharin.: "Bactericidal activity of C9ーdeficient human serum."

    • Related Report
      1989 Annual Research Report
  • [Publications] 洪卿秀(Kyongsu.Hong): "H因子(factor H)(BetaIHグロブリン)(BetaIH)" 日本臨床, 6 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 洪卿秀(Kyongsu Hong): "臨床免疫(V補体 H因子)" 日本臨床, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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