Cloning and sequence of MS fimbrial gene of Serratia marcescens.
| Project/Area Number |
63570196
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
細菌学
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
MORIYA Tetsuhiro Kyushu University, Medicine, Research Associate, 医学部, 助手 (10140790)
|
|---|
| Project Period (FY) |
1988 – 1989
|
| Project Status |
Completed (Fiscal Year 1989)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | Serratia marcescens / Fimbriae / Cloning / Monoclonal antibody |
|---|
| Research Abstract |
The fimbriae of Serratia marcescens were roughly classified into three types (SMF-1, -2 -3), which were mannose-sensitive- (MS-), mannose-resistant- (MR-) and non-hemagglutinative-fimbriae, according to the difference of their hamagglutination patterns or agglutination patterns with antibodies raised against the fimbriae of S. marcescens. I found two different MS fimbriae (SMF-4, -5) in S. marcescens, whose structure or molecular size of the fimbrial subunits were different from that of SMF-1 though they had the common antigenicity with SMF-1, .
Analysis of these fimbrial genes, which were cloned into Escherichia coli respectively, indicated that their gene organization were resemble to that of E. coli.
I confirmed that smf-3 was not expressed in the parent strain US5 and was expressed only after it was moved into E. coli. The subunit of SMF-3 seemed to have unique structure because it had contained no system. Though no clinical isolates of S. marcescens were agglutinated with the antibody raised against SMF-3, about 50% strains of them showed positive reaction by Southern hybridization with the four Sal I DNA fragments probes which contained the part of smf-3. Indeed, smf-3 could be cloned into E. coli from one hybridization-positive strain other than US5.
I have determined the DNA sequence of the major structural fimbrial subunit genes (smf-1, -2 and -3). After determination of DNA sequence of smf-4 and smf-5 I would investigate the amino acids sequence responsible to antigenicity and morphorogical formation of the fimbriae by using the data of their DNA or amino asids sequence.
|
Report
(2 results)
Research Products
(18 results)
