| Project/Area Number |
63570227
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
SATO Hidetoshi Juntendo Univ., Pathology, Associate Professor, 医学部, 助教授 (30091573)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Hiroyuki Juntendo Univ., Pathology, Instructor, 医学部, 助手 (60189313)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | autoimmune disease / activated B cell / monoclonal antibody / lymphokine receptor / cDNA cloning / 活性化B細胞抗原 / B細胞亜集団 / 増殖分化因子リセプタ- / アフィニティフグロマトグラフィ / 免疫グロブリンス-パ-ジ-ンファミリ- / ウエタンブロット / アミノ酸配列分析 / モノクロナール抗体 |
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| Research Abstract |
The lack of useful markers for the detection of activated B cells in mice has hampered the progress in studies on the cellular basis of autoantibody synthesis. Recently, we established a monoclonal antibody, Lp-3, which detects an early activation antigen on murine B cells, by immunizing a rat with proliferating lymph node cells from MRL-lpr/lpr mice. The results of analyses of Lp-3 antigen are as follows: 1) Although the antigen Lp-3 was constitutively expressed on granulocytes and T cells, the expression was not found on resting B cells. 2) Lp-3 could be induced on one-half of mitogen-stimulated B cell population at early activation phases of cell cycle. 3) There was an elevated population of B cells with a bright Lp-3 expression in the spleen of autoimmune-prone (NZB X NZW)F1 mice. Such Lp-3^+ B cell subpopulation was induced by IL-5 and IL-6 to produce IgM and IgG anti-DNA antibodies, respectively, suggesting that LP-3 may be a receptor- associated molecule for a certain activation or differentiation factor. 4) Lp-3 was found to be a single polypeptide chain, having molecular mass of 132 Kd. 5) Data of partial amino acid sequences showed that Lp-3 has a domain homologous to immunoglobulin superfamily. 6) Analysis of nucleotide sequence is ongoing using several cDNA clones for Lp-3.
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