Structure and function of Lp-3, an early activation antigen on murine B cells
Project/Area Number |
63570227
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Immunology
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Research Institution | Juntendo University School of Medicine |
Principal Investigator |
SATO Hidetoshi Juntendo Univ., Pathology, Associate Professor, 医学部, 助教授 (30091573)
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Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Hiroyuki Juntendo Univ., Pathology, Instructor, 医学部, 助手 (60189313)
|
Project Period (FY) |
1988 – 1989
|
Project Status |
Completed (Fiscal Year 1989)
|
Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
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Keywords | autoimmune disease / activated B cell / monoclonal antibody / lymphokine receptor / cDNA cloning / 活性化B細胞抗原 / B細胞亜集団 / 増殖分化因子リセプタ- / アフィニティフグロマトグラフィ / 免疫グロブリンス-パ-ジ-ンファミリ- / ウエタンブロット / アミノ酸配列分析 / モノクロナール抗体 |
Research Abstract |
The lack of useful markers for the detection of activated B cells in mice has hampered the progress in studies on the cellular basis of autoantibody synthesis. Recently, we established a monoclonal antibody, Lp-3, which detects an early activation antigen on murine B cells, by immunizing a rat with proliferating lymph node cells from MRL-lpr/lpr mice. The results of analyses of Lp-3 antigen are as follows: 1) Although the antigen Lp-3 was constitutively expressed on granulocytes and T cells, the expression was not found on resting B cells. 2) Lp-3 could be induced on one-half of mitogen-stimulated B cell population at early activation phases of cell cycle. 3) There was an elevated population of B cells with a bright Lp-3 expression in the spleen of autoimmune-prone (NZB X NZW)F1 mice. Such Lp-3^+ B cell subpopulation was induced by IL-5 and IL-6 to produce IgM and IgG anti-DNA antibodies, respectively, suggesting that LP-3 may be a receptor- associated molecule for a certain activation or differentiation factor. 4) Lp-3 was found to be a single polypeptide chain, having molecular mass of 132 Kd. 5) Data of partial amino acid sequences showed that Lp-3 has a domain homologous to immunoglobulin superfamily. 6) Analysis of nucleotide sequence is ongoing using several cDNA clones for Lp-3.
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Report
(3 results)
Research Products
(11 results)
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[Publications] Sekigawa,I.,Noguchi,K.,Hasegawa,K.,Hirose,S.,Sato,H.& Shirai,T.: "B cell hyper-responsiveness to IL2 and the age-associated decline in murine lupus." Clin.Immunol.Immunopathol.51. 172-184 (1989)
Description
「研究成果報告書概要(和文)」より
Related Report
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[Publications] Hasegawa,K.,Abe,M.,Okada,T.,Hirose,S.,Sato,H.& Shirai,T.: "Are Lyl B cells responsible for the IL2-hyperresponsiveness of B cells in autoimmune-prone NZB × NZW F1 mice?" International Immunol.1. 99-101 (1989)
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Publications] Abe,M.,Okada,T.,Matsumoto,K.,Ishida,Y.,Shiota,J.,Nishimura,H.,Hirose,S.,Sato,H.& Shirai,T.: "The novel murine B cell differentiation antigen Lp-3." International Immunol.1. 576-581 (1989)
Description
「研究成果報告書概要(和文)」より
Related Report
-
[Publications] Sekigawa, I., Noguchi, K., Hasegawa, K., Hirose, S., Sato, H. & Shirai, T.: "B cell hyper-responsiveness to IL2 and the age-associated decline in murine lupus." Clin. Immunol. Immunopathol. 51: 172-184, 1989.
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Publications] Hasegawa, K., Abe, M., Okada, T., Hirose, S., Sato, H. & Shirai, T.: "Are Lyl B cells responsible for the IL2-hyperresponsiveness of B cells in autoimmune-prone NZB X NZW F1 mice?" International Immunol. 1:99-101, 1989.
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Publications] Abe, M., Okada, T., Matsumoto, K., Ishida, Y., Shiota, J., Nishimura, H., Hirose, S., Sato, H. & Shirai, T.: "The novel murine B cell differentiation antigen Lp-3." International Immunol. 1: 576-581, 1989.
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Publications] Sekigawa,I.,Noguchi,K.,Hasegawa,K.,Hirose,S.,Sato,H.& Shirai,T.: "B cell hyper-responsiveness to IL2 and the age-associated decline in murine lupus." Clin.Immunol.Immunopathol.51. 172-184 (1989)
-
[Publications] Hasegawa,K.,Abe,M.,Okada,T.,Hirose,S.,Sato,H.& Shirai,T.: "Are Lyl B cells responsible for the IL2-hyperresponsiveness of B cells in autoimmune-prone NZB X NZW F1 mice?" International Immunol.1. 99-101 (1989)
-
[Publications] Abe,M.,Okada,T.,Matsumoto,K.,Ishida,Y.,Shiota,J.,Nishimura,H.,Hirose,S.,Sato,H.& Shirai,T.: "The novel murine B cell differentiation antigen Lp-3." International Immunol.1. 576-581 (1989)
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