| Project/Area Number |
63570231
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
MIYASAKA Masayuki Tokyo Metropol.Inst.Med.Sci., Dept.Imm., Head, 免疫研究部門, 室長 (50064613)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Kazuhito Tokyo Metropol.Inst.Med.Sci., Dept.Imm., Temp.Research member, 免疫研究部門, 非常勤研究員 (40034988)
KOTANI Masahary Tokyo Metropol.Inst.Med.Sci., Dept.Imm., Research member, 免疫研究部門, 研究員 (10195737)
TAMATANI Takuya Tokyo Metropol.Inst.Med.Sci., Dept.Imm., Research member, 免疫研究部門, 研究員 (70207231)
KITAMURA Fujiko Tokyo Metropol.Inst.Med.Sci., Dept.Imm., Research member, 免疫研究部門, 研究員 (90124453)
佐藤 功栄 (財)東京都臨床医学総合研究所, 免疫研究部門, 研究員 (40124458)
通堂 満 (財)東京都臨床医学総合研究所, 免疫研究部門, 研究員 (40197645)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Lymphocyte / Lymphocyte recirculation / Homing / Endothelial cell / Cell adhesion / Adhesion molecule / Immunomodulation / 接着因子 / ホーミング |
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| Research Abstract |
In order to identify molecules involved in lymphocyte recirculation, particularly interactions between lymphocytes and HE (high endothelial) cells, we tried successfully to establish cultured cell lines derived from rat high endothelial venules (HEV). We then developed an in vitro assay with which to quantitate lymphocyte interaction with HE cells, a most important process in lymphocyte recirculation. We have made number of attempts to generate monoclonal antibodies (MAb) which can block this heterocellular interaction, and have subsequently obtained two MAb, one of which recognizes the rat ICAM-1, the other the rat LFA-1. By the use of the in vitro assay described above, we investigated the extent of contribution of the LFA-1 and ICAM-1 molecules in lymphocyte-HEV interaction. We have demonstrated that; (1) neither LFA-1 nor ICAM-1 plays a significant role in interaction between resting lymphocytes and HEV, (2) when lymphocytes are activated, LFA-1 dependent adhesion pathways becomes operative, although, depending on the extent of activation, ICAM- 1 dependent adhesion pathway is differentially used in the interaction between lymphocytes and HE cells. Further attempts are being made to identify molecules directly involved in lymphocyte-HE cell interaction.
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