The Distribution of the Blood Group A- and B-active Glycolipids in the Human Erythrocyte Membrane
| Project/Area Number |
63570276
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
UCHIMA Eikoh University of the Ryukyus, School of Medicine, Department of Legal Medicine, Instructor, 医学部, 助手 (90093377)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1989: ¥200,000 (Direct Cost: ¥200,000)
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| Keywords | Blood groups / Glycolipids / Immunostaining / Monoclonal antibody / Chromatoscanner / モノクローナル抗体 |
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| Research Abstract |
A quantitative study on the distribution of structural variations of the A- and/or B-active glycolipids in human blood group A, B and AB erythrocyte membranes was performed. In order to detect the glycolipids with a blood group specificity, immunostaining on a thin-layer chromatography plate, using a monoclonal antibody, was applied, and the relative antigenic ratio of each glycolipid was determined by the use of a chromatoscanner.
The results indicated that the blood group active glycolipids with shorter and simpler carbohydrate chains were found to have a greater distribution on the erythrocyte membranes than those with longer and more complex carbohydrate chains. Further, it was found that the antigenic ratios of four A-active glycolipids from the blood A and AB erythrocyte membranes were similar, and that this also was observed with regard to the six B-active glycolipids from the blood group B and AB erythrocytes.
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Report
(3 results)
Research Products
(4 results)
