| Project/Area Number |
63570281
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
SAITO Yasushi Chiba University, Second Department of Internal Medicine, Lecturer, 医学部, 講師 (50101358)
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| Co-Investigator(Kenkyū-buntansha) |
MORISAKI Nobuhiro Chiba University, Second Department of Internal Medicine, Associate, 医学部, 助手 (40174411)
SHIRAI Kohji Chiba University, Second Department of Internal Medicine, Associate, 医学部, 助手 (00150269)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Endothelial Cell / Smooth Muscle Cell / Phenotype / Growth Factors / Phenotype / 増殖因子 / 中膜平滑筋細胞 / マクロファージ / 低比重リポ蛋白 |
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| Research Abstract |
The aim of this project is to clarify the factor to produce the intimal smooth muscle cell (SMC) because the intimal thickening, which is constantly observed in atheromatous lesions, is mainly consist of the intimal SMC. It is generally accepted that intimal SMC might be derived from the inedial SMC by migration and the SMC is enhanced to grow by some growth factors. In these process, the endothelial cell is considered to play an important role. Therefore, we tried to examined the effect of endothelial cell (EC) on the SMC growth using the EC-SMC co-culture system. The system was established and the results are as follows.
1) Co-cultured SMC growth with EC was inhibited but slightly increased in the presence of Iridoinethasine. 2) Conditioned medium with EC enhanced the SMC growth in the growing phase more than in the confluent phase. 3) The SMC secreted the SDGF (smooth muscle derived growth factor) when the SMC was cultured with the conditioned medium. 4) The growth stimulating activity from the EC was inhibited by the addition of anti-PDGF antibody. 5) PDGF itself stimulates the SDGF secretion from SMC.
Above these results, we concluded that EC modulated the SMC phenotype and one of the factors from EC to change the SMC phenotype might be PDGF.
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