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The role of anionic charge of the glomerular basement membrane.

Research Project

Project/Area Number 63570288
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

HISHIDA Akira  Hamamatsu University School of Medicine Internal Medicine, Assistant Professor, 医学部, 講師 (70111812)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Shuzo  Hamamatsu University School of Medicine Internal Medicine, Assistant Research As, 医学部, 助手 (60195782)
YAMAMOTO Tatsuo  Hamamatsu University School of Medicine Internal Medicine, Assistant Research As, 医学部, 助手 (30200819)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsglomerular basement membrane / anionic charge barrier / proteoglycan / cuprolinic blue / aminonucleoside nephrosis / ネフローゼ
Research Abstract

Despite the growing number of attempts to evaluate anionic site in glomerular basement membrane (GBM), there has not been an established method which accurately quantifies an alteration of the anionic charge of the GBM. The present work was performed to develop a method to quantify the anionic charge of the GBM and to estimate the change of the charge barrier in puromycin aminonucleoside (PAN) nephritis in rats. Cuplorinic blue which contains copper was used as a cationic probe. Left kidney was perfused with sodium acetate buffer containing cuprolinic blue. After perfusion, GBM was isolated by sieving technique and the copper content was measured with an atomic absorption spectrophotometer. Electron microscopic study of isolated GBM revealed the dye-positive fibrils on both side of the lamina densa. Isolation procedure, such as sonication did not affect the copper content of GBM. The degree of copper binding to GBM was markedly reduced in PAN nephritis rats. The degree of copper binding to GBM after PAN injection was markedly reduced at day 9 and returned to the control level at day 42, concomitantly with the recovery of albuminuria. In vito study using isolated GBM, the effects of pH on the cuprolinic blue binding was studied. At pH between 8.0 and 4.0, no significant difference was observed in the cuprolinic blue binding to GBM, but at pH between 3.0 and 4.0, the binding rate began to decrease. This pH-dependent dissociation curve of cuplrolinic blue suggested that the dye preferentially reacted with the sulfate groups in GBM. These results demonstrated that the method which we developed may be useful to quantifies an alteration of the anionic charge of GBM.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Fujigaki,S.Kobayashi,M.Nagase,Y.Muranaka,N.Honda: "Alteration of glomerular basement membrane relevant to hematuria." Virchow's Archives of Pathology. 413. 159-165 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Yoneyama,S.Kobayashi,Y.Fujigaki,M.Nagase,N.Honda: "Immunoelectron microscopic study of intraglomerular fibronectin distribution in rat experimental glomerulonephritis." J.Clin.Electron Microscopy. 21. 934-935 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Fujigaki,S.Kobayashi,T.Yoneyama,M.Nagase,N.Honda: "Time sequential study of the migration of immune complex from subendothelial to subepithelial space of the GBM." J.Clin.Electron Microscopy. 21. 936-937 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小林修三、池谷直樹、新井隆己、長瀬光昌、本田西男、村中祥悟: "実験的膜性腎症における糸球体基底膜陰荷電物質の超微形態学的変化" 臨床電子顕微鏡学会誌. 21. 61-69 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Kobayashi: "Analysis of anionic charge of the glomerular basement membrane in aminonucleoside nephrosis." Kidney International. 35. 1405-1408 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shuzo Kobayashi, Mitsumasa Nagase, Nishio Honda, Kyoko Adachi, Norio Ichinose, and Akira Hishida: "Analysis of anionic charge of the glomerular basement membrane in aminonucleoside nephrosis." Kidney International, 35, 1405-1408, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tatsu yamamoto, Mitsumasa Nagase, and Nishio Honda: "Inhibitory effect of lysozyme on the intraglomeular immune complex formation in lupus mice." Clinical Immunology and Immunopathology, 47, 27-38, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shuzo Kobayadhi: "Analysis of anionic charge of the glomerular basement membrane in aminonucleoside nephrosis." Kidney International. 35. 1405-1408 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Fujigaki;S.Kobayashi;M.Nagase;Y,Muranaka;N.Honda: Virchow's Archives of pathology. 413. 159-165 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Yoneyama;S.Kobayashi;Y.Fujigaki;M.Nagase;N.Honda: J.Clin.Electron Microscopy.

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Fujigaki;S.Kobayashi;T.Yoneyama;M.Nagase;N.Nonda: J.Clin.Electron Microscopy.

    • Related Report
      1988 Annual Research Report
  • [Publications] 小林修三、池谷直樹、新井隆己、長瀬光昌、本田西男、村中祥悟: 臨床電子顕微鏡学会誌. 21. 61-69 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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