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Transformational Alteration of Human pancreatic Gamma-Glutamyl-transpeptidase (gamma-GTP)

Research Project

Project/Area Number 63570318
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionKanazawa University

Principal Investigator

SAWABU Norio  Kanazawa University, Cancer, Research Institute, Professor, がん研究所, 教授 (90019969)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsgamma-GTP / Pancreatic Cancer / Sugar chain / Hepatocellular Carcinoma / Monoclonal antibody / Transformational alteration / 糖鎖構造 / γ-glutamyl transpeptidase(γ-GTP)
Research Abstract

To elucidate the specific changes of pancreatic gamma-glutamltranspeptidase (gamma-GTP) associated with malignant transformation, some properties of gama-GTP purified from pancreatic cancer were compared with those of gamma-GTPs from normal pancreas and other tissues. Four of five pancreatic cancer gamma-GTPs showed distinctly slower electrophoretic mobility than the normal enzymes. Isoelectric points of pancreatic cancer gamma-GTPs varied in each case, but all of them were higher than those of normal enzymes. This difference in isoelectric points of gamma-GTPs between cancerous tissue and normal tissue was reduced by neuraminidase treatment. Lectin affinity chromatography revealed two of five pancreatic cancer gamma-GTPs with a greater affinity to concanavalin A (Con A) than normal pancreas gamma-GTPs. Four of five pancreatic cancer gamma-GTPs had a greater affinity to Lens culinaris agglutinin (LCA) than normal pancreas gamma-GTPs. Normal pancreas had little affinity to phaseolus vul … More garis erythroagglutinating (E-PHA), but two of five pancreatic cancer gamma-GTPs had an apparent affinity to E-PHA and one of them had a slight affinity to E-PHA. These results indicate that the transformational changes of pancreatic cancer gamma-GTP are mainly induced in the sugar chains of the enzyme molecules resulting in a lower content of sialic acid and higher content of fucose and bisecting GlcNAc residue as compared with the normal pancreatic enzymes.
Furthermore, to develop the monoclonal antibody (MAb) that recognize the specific alteration of sugar chain of pancreatic cancer gamma-GTP, we prepared the MAb against gamma-GTP from pancreatia cancer using the hybridoma technique. All of the MAbs were reactive with gamma-GTPs from not only pancreatic cancers but also other tissues, and could not recognize sugar chain but peptide portion of gamma-GTP. This is considered to be due to the fact that the immunogenicity of sugar chain can be weaker in comparison with that of peptide portion. We are now preparing the specific MAb, considering a new device. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 澤武紀雄ほか: "膵癌の腫瘍マ-カ-" 内科. 62. 244-249 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 太田秀樹: "Tumor markerによるfollow-upーーγ-GTP" 日本臨床. 47. 1114-1118 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 澤武紀雄ほか: "消化器癌における癌性糖鎮抗原" 臨床病理. 37. 509-516 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Ohta,et al.: "Characterization on of γ-GTP from human pancreatic cancer" Pancreas. 5. 82-90 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Satomura,et al.: "Expressin of various sialylated carbohydrate antigen in malignant and non-malignant pancreatic tissues" Pancreas.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Ohta,et al.: "Characterization of γ-GTP from hepatocellular carcinoma, and comparison with the enzymes from normal liver cirrhotic liver and other tissues" Hepatology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Sawabu, et al: "Cancer-associated carbohydrate antigens which are available to serum diagnosis" Jpn. J. Cancer Chemother, 15(4) : 1091-1101, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Sawabu, et al: "Tumor markers of pancreatic cancer" Int. Med, 62(2): 244-249, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Ohta, et al: "Follow-up by tumor marker-gamma-GTP" Jpn. J. Clin. Med, 47(suppl): 1114-1118, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Sawabu, et al: "Present aspect of tumor markers for pancreatic cancer" Clin. Gastroneterol, 86(5): 640-649, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Kawakami, et al: "Clinical evaluation of serum sialyl SSEA-1 (SLX) in diagnosis of cancer" Jpn. J. Gastroenterol, 86(5): 1141-1148, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Ohta, et al: "Characterization of gamma-GTP from human pancreatic cancer" Pancreas, 5(1): 82-90, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Satomura, et al: "Expression of various sialylated carbohydrate antigens in malignant and nonmalignant pancreatic tissue" Pancreas.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Ohta, et al: "Characterization of gamma-GTP from hepatocellular carcinoma, and comparison with the enzyme from normal liver, cirrhotic liver and other tissues" Hepatology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 太田英樹ほか: "Tumor markerによるfollow-up--γ-GTP" 日本臨床. 47. 1114-1118 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 澤武紀雄ほか: "膵腫瘍マ-カ-の現状" 消化器科. 10. 640-649 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 澤武紀雄ほか: "消化器癌における癌性糖鎖抗原" 臨床病理. 37. 509-516 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideki Ohta,et al.: "Characterization of γ-GTP from human pancreatic cancer" Pancreas. 5. 82-90 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshitaka Satomoura,et al.: "Expression of various sialylated carbohydrate antigen in malignant and non-malignant pancreatic tissues" Pancreas.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideki Ohta,et al.: "Characterization of γ-GTP from hepatocellular carcinoma、and comparison with enzymes from normal liver、cintrotic liver and other tissues" Hepatology.

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田英樹 他: 第7回腫瘍マーカー研究会プロシーデングス. 3. 220-222 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 澤武紀雄 他: クリニカ. 15. 574-579 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 澤武紀雄 他: 内科. 62. 244-249 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 太田英樹 他: 図説臨床「癌」シリーズ腫瘍マーカー. 25. 21-24 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 太田英樹 他: 日本臨床. 47. 1114-1118 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hideki,Ohta.et al: Pancreatolog.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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