| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Eosinophilia in peripheral blood and eosinophil infiltration in the lesion of the lung of the patients with respiratory diseases such as bronchial asthma, PIE syndrome, or allergic rhinitis are often seen clinically. In this study, we observed the release of eosinophil colony stimulating factor (E-CSF), which is thought to be play important role in eosinophilia by the ability to stimulate eosinophil proliferation, from lymphocytes. Also we investigated the activity of E-CSF and neutrophil chemotaxisis in the pleural fluid from patients with PIE syndrome.
First, we measured E-CSF activity in the pleural fluid, and found the relationship between the E-CSF activity and eosinophil number in the fluid of the patients with PIE syndrome. The nature of the E-CSF in the fluid showed heat resistant and a molecular weight of about 50,000. In pleural fluid, neutrophil chemotactic factor also was detected. These data indicated that eosinophilia in the pleural fluid of patients with PIE syndrome is related to the appearance of E-CSF and neutrophil chemotactic factors locally.
From the experiments about peripheral blood lymphocytes, we showed that mononuclear cells, especially T-lymphocytes, released E-CSF. And the release of E-CSF was stimulated by IL-2, PHA and antigens such as housedust, but was suppressed by prednisolone or FL-506, one of immunosuppresive agent. These data indicated that T-lymphocytes can release E-CSF by various stimuli, and is thought to be related to the eosinophilia of patients with pulmonary diseases.
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