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Antineural Autoantibodies in Patients With Paraneoplastic Cerebellar Degeneration

Research Project

Project/Area Number 63570358
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionFukushima Medical College (1989)
Tohoku University (1988)

Principal Investigator

TSUKAMOTO Tetsuro  Fukushima Medical College, Dept Neurology, Assist Prof., 神経内科, 講師 (20171978)

Co-Investigator(Kenkyū-buntansha) YOSHIE Osamu  Shionogi Co., Director, 研究部, 部長 (10166910)
YAMAMOTO Teiji  Fukushima Medical College, Dept Neurology, Prof., 神経内科, 教授 (10106487)
IWASAKI Yuzo  Tohoku Uni Sch Med., Dept Neurol Sci., Prof., 医学部・病態神経学, 教授 (00142927)
Project Period (FY) 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsParaneoplastic cerebellar degeneration / Antineural autoantibody / Malignant tumor / Anti-Purkinje cell antibody / cDNA cloning
Research Abstract

We studied serum autoantibodies from six patients with paraneoplastic cerebellar degeneration using rat brains. Immunohistochemically, serum samples from four of six patients with uterine or overian tumors showed a similar staining pattern, labeling Purkinje cells and various other neurons distributed throughout the brain and spinal cord. The immunoblot study showed that these serum samples recognized the common 52- kd band. Serum from a patient with duct carcinoma of the breast reacted with the 46- kd band with a similar but weaker immunohistochemical reactivity. Serum from another patient with small-cell carcinoma of the lung in whom paraneoplastic cerebellar degeneration and Eaton-Lambert myasthenic syndrome developed recognized the 40-kd and 38-kd bands with a different imunostaining pattern. Autoantibodies in patients with paraneoplastic cerebellar degeneration are directed to a variety of neuronal antigens that may differ from patient to patient.
We isolated a cDNA clone encoding … More a 52-kd protein recognized by an anti-Purkinje cell antibody in the serum from a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. The recombinant protein expressed in prokaryotic cells was specifically recognized by the anti-Purkinje cell antibody from the patient and its molecular weight was identical with that of antigenic protein in the cerebellum. The deduced protein consisted of 450 amino acids dominated by hydro- philic residues, the calculated relative molecular mass was 51238 and the predicted value of the isoelectric point was 4.99. This cDNA sequence and the deduced protein sequence have not been reported previously and showed no homologies with the cDNA or the amino acid sequences in the GenBank, EMBL or NBRF databases, including the cDNA for a 34-kd cerebellar protein (CDR34) which is recognized by an anti-Purkinje cell antibody. Unexpectedly, the transcript of this gene was detected not only in the cerebellum and the brain stem, but also in an extraneural tissue, the intestine. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Tetsuro Tsukamoto: "Antineural autoantibodies in patients with paraneoplastic cerebellar deyeneration" Arch Neurol. 46. 1225-1229 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Koichiro Sakai: "Isolation of a cDNA clone encoding an autoantgen recognited by an anti-Purkinje cell antibody from a patient with poraneoplastic cerebellar degeneration" Ann Neurol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Tsukamoto et al.: "Neuroimmunological Diseases" University of Tokyo Press, 413 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsukamoto, T., Yoshie, O., Tada, K., Iwasaki, Y.: "Anti-Purkinje cell antibody producing B-cell lines from a patient with paraneoplastic cerebellar degeneration." Arch Neurol 44:833-837, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsukamoto, T., Iwasaki, Y., Yoshie, O., Yamamoto, H., Suzuki, Y.: "Anti-Purkinje cell antibody producing B-cell lines from a patient with paraneoplastic cerebellar degeneration." "Neuroimmunological Diseases" ed. by Igata, A., University of Tokyo Press, Tokyo. 347-352 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsukamoto, T., Yamamoto, H., Iwasaki, Y., Yoshie, O., Terunuma, H., Suzuki, H.: "Antineural autoantibodies in patient with paraneoplastic cerebellar degeneration." Arch Neurol 46:1225-1229, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakai, K., Mitchell, D.J., Tsukamoto, T., Steinman, L.: "Isolation of a cDNA clone encoding an autoantigen recognized by an anti-Purkinje cell antibody from a patient with paraneoplastic cerebellar degeneration." Ann Neurol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tatsuro Tsukamoto: "Antineural autoantibodies in patients with paraneoplastic cerebellar degeneration" Arch Neurol. 46. 1225-1229 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Koichiro Sakai: "Isolation of a cDNA clone encoding an autoantigen recognited by an anti-Purkinje cell antibody from a patient with poraneoplastic cerebellar degeneration" Ann Neurol.

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Hisatomo.;T.Tsukamoto.;H.Aso.;K.T amura.: J .Neurol.Sci.85. 259-266 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Tsukamoto.;H.Yamamoto.;Y.Iwasaki.; O.Yoshie.et al.: Arch Neurol.

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Tsukamoto.;Y.Iwasaki.;O.Yoshie.;H .Yamamoto.;H.Suzuki: "Neuroimmundogical Diseases." University of Toky o Press, 347-352 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Tsukamoto.;Y.Iwasaki.;M.Osame.;A.I gata.: "Neuroi mmundogical Diseases." University of Tokyo Press, 39-40 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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