| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
To elucidate the role of noradrenergic system in the seizures of epileptic E1 mice, we tested the anticonvulsant effects of various drugs. We also quantitated adrenergic receptors, noradrenaline (NA), dopamine (DA) and cyclic-AMP (cAMP).in the whole brains and regional brains of the stimulated and unstimulated E1 mice, and their maternal ddY mice. Obtained results are as followings;
1) NAergic system in the brains of E1 mice seemed to play a inhibitory bole on the seizures of the E1 mice. This inhibition seemed to be mediated by activations of alpha_1 - and alpha_2- adrenoceptors.
2) Up-regulations of alpha_1-adrenoceptors in the striatum and cerebellum, alpha_2-adrenoceptors in the cerebral cortex, hippocampus and brainstem were observed in the stimulated and unstimulated E1 mice.
3) Decreases of NA contents were observed in the striatum, hippocampus and frontal cortex of the stimulated E1 mice. These decrebtses became to be observed when mice were getting seizure susceptibility.
4) From the results of NA turnover and time-dependent change of NA contents after seizures, hyperfunction of NAergic neurons in the hippocampus and hyperfunction of those in the striatum were suggested. Therefore, the manner of inhibition for seizured by NA- ergic system was different by brain regions, and this difference may be closely related the seizure susceptibility of the E1 mice.
5) In the cerebral cortex of the stimulated E1 mice, c-AMP concentrations were decreased. After seizures, c-AMP contents increased in all of the brain regions except for hippocampus.
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