| Project/Area Number |
63570452
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE |
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Principal Investigator |
TAKEUCHI Yoshihiro KYOTO PREFECTURAL UNIVERSITY OF MEDICIN, RESEARCH ASSOCIATE, 医学部, 助手 (80188169)
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| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Masashi KYOTO PREFECTURAL UNIVERSITY OF MEDICIN, RESEARCH ASSOCIATE, 医学部, 助手 (60188795)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Seizure / Serotonin / Mouse / Neostriatum / Immunohistochemistry / 痙攣 |
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| Research Abstract |
Serotonin neurons in the brainstem give rise to highly branched fibers which innervate virtually the entire central nervous system, comprising the most expatisive neuronal network among other neurotransmitters. The aim of this project is to examine changes occurring in the serotonin neuron system in experimentally induced convulsions by means of a modified immunoperoxidase method. To obtain quantitative data on the density or serotonergic innervation, the total length of serotonin-immunoreactive fibers was calculated using the MOP system. A marked increase in serotonin immunoreactivity was observed at the rostral and intermediate levels or the neostriatum of mice kept at high temperature (50ﾟC) with no convulsions, and a significant reduction in serotonin immunoreactivity was verified throughout the neostriatum of mice which had hyperthermia-induced seizures. in the case or pilocarpine-induced convulsions, a significant reduction in serotonin immunoreactivity was observed at the rostral and caudal levels of the neostriatum or mice which had generalized seizures due to the systemic administration of pilocarpine (350 mg/kg). In both experimental convulsion models, the serotonin immunoreactivity remained unchanged in the paleostriatum and neocortex and the degree of serotonin reduction in the neostriatum was significantly greater in hyperthermia-induced convulsions than that in pilocarpine-induced convulsions. These results suggest that striatal serotonin may be an important mediater in the mechanism of convulsions and that more precise regional analysis using quantitative histochemical methods is essential for elucidation of the mechanism underlying human neurological disorders.
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