• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The Role of Ras Oncogene Product p21 in Human thyroid tissue

Research Project

Project/Area Number 63570545
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionKitasato University

Principal Investigator

ABE Yoshifumi  Kitasato University, School of Medicine Assistant Professor, 医学部, 講師 (30124928)

Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsThyroid carcinoma / ras oncogene / GTP binding protein / 癌遺伝子 / トランスメンブレンコントロ-ル / 細胞内カルシウム / トランスメンブレンコントロール
Research Abstract

Recent data suggest that the ras oncogene products modulate the responsiveness of several enzymes in the cell membrane that respond to the extercellular singnels.
The membrane fraction were freshly prepared from six human thyroid carcinomas and adjacent normal thyroid tissues. Western blotting analysis was used to determine the amount of p21 (ras oncogene product) in membrane fraction using specific antisera (NCC-RAS-004). Levels of Gi subunit were monitored by labeling with [^<32>P] NAD and pertussis toxin. The adenyalte cylase activities were measured by RIA of the cyclic AMP generated during 30 min incubation of the membrane fraction in the presence of 10mU/ml TSH. In western blotting analysis, the monoclonal antibody NCC-RAS-004 detected clear band of p21 in membranes from both normal and carcinoma thyroid tissues. When the staining intensity of bands was examined with a densitometer, the intensity of the p21 band of carcinomas tissues was stronger by two-fold or more compared with … More that of adjacent normal thyroidas in 5 of 6 cases. No abnormally migrating p21 was detected in these cases. In agreement with other studies, pertussis toxin labeled a 41KDa band (Gi) in both normal and carcinoma thyroid membranes. However the membranes from carcinomas reduced the level of labeling of Gi by 50-10% in 5 of 6 cases. The level of enhanced expression of p21 were significantly correlated with the level of increased response of the adenylate cyclase to TSH and inversely related to the level of Gi assessed by ADP-ribosylation with petrussis toxin.
Our results suggest that the ras oncogene product P21 play enhancing role in adenylate cyclase system and its modulation by the G protein. Thus a ras producing cell is hypersensitive to a variety of hormones and growth factors and this hypersensitivity might contribute significantly to the oncogenicity of these proteins under physiological conditions in the whole animal. Further studies are required to clarify whether the changes in sensitivity of adenyalte cyclase activation to TSH are mediated ras oncogene or its products. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 阿部 好文: "ヒト甲状腺組織における癌遺伝子rasの産物とその機能" Modern Physician. 8. 1758-1763 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshifumi Abe: "the role of ras onoogene prodnct p21 in human thyroid tissne" The Kitasato Archires of Experimental Medicine. 8. 72-74 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Akira Kanamori: "Epidermal Growth factor receptor in plasma membranes of normal and diseased human thyroid glands" J Clin Endocrinol Metab. 68. 899-903 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 阿部 好文: "ヒト甲状腺細胞におけるラスがん遺伝子の発現と遺伝子産物p21の機能に関する研究" ホルモンと臨床. 37. 121-128 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 阿部 好文: "ヒト甲状腺癌の増殖とホルモンによるその制御癌遺伝子の発現と情報受容伝達機構の異常について" 臨床成人病. 19. 1787-1788 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toru Kaneya: "Discordant secretion of calcitonin and chromogranin in the human medullary thyroid carcinoma cell line" Endocr Pathol. 2. 16-23 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Abe, A. Kanamori and Y. Yajima: "The role of ras oncogene product p21 in human thyroid tissue" The Kitasato Archives of Medicine Experimental. 62(1). 72-74 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Abe, A. Kanamori, Y. Yajima, Y. Memabe and K, Ito.: "The role of ras oncogene product p21 in human thyroid carcinoma" Modern Physician. 8(12). 1758-1763 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A. Kanamori. Y. Abe, Y. Yajima, Y. Manabe and K. Ito: "Epidermal Growth Factor Receptorsin Plasma Membranes of Normal and Diseased Human Thyroid Glands" J Clinical Endocrinol Metab. 68(5). 899-903 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kameya, K. Kadoya, H. Iguchi, Y. Abe and K. Shimaika.: "Discordant secretion of calcition and chromogranin in the human medullary thyroid carcinoma cell line" Endocrine Pathology. 2. 16-23 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 阿部 好文: "非機能性甲状腺腫瘍の生化学的特性ーAFTNとの比較を中心にー" 医学のあゆみ. 153. 8-12 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 阿部 好文: "ホルモン産生腫瘍 ー診断のポイントー" 図説臨床[癌]シリ-ズ 癌とホルモン. 32. 133-141 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toru Kameya: "Discordant secretion of calcitonin and chromogranin in the human medullary thyroid carcinoma cell line" Endocrine Pathology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshifumi Abe: "The role of ras oncogene product p21 in human thyroid tissne" Endocrinology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshifumi Abe: "The role of ras oncogene product p21 in human thyroid tissue" The Kitasato Archives of Experimental Medicine. 62. 72-74 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akira Kanamori: "Epidermal growth factor receptors in plasma membranes of normal and diseased human thyroid glands" J Clinical Endocrinology and Metabolism. 68. 899-903 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 阿部好文: "ヒト甲状腺細胞におけるラスがん遺伝子の発現と遺伝子産物p21の機能に関する研究" ホルモンと臨床(秋季増刊). 37. 121-128 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 阿部好文: "培養によるヒト甲状腺細胞機能の研究-甲状腺癌細胞の情報受容伝達機構の異常-" Human cell. 2. 238-245 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 阿部好文: "ヒト甲状腺癌の増殖とホルモンによるその制御-癌遺伝子の発現と情報受容伝達機構の異常について" 臨床成人病. 19. 1787-1788 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 阿部好文: 日本臨床代謝学会記録. 25. 160-161 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 阿部好文: 臨床病理. 36. 1358-1363 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 阿部好文: Modern Physician. 8. 1758-1763 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akira Kanamori: The Journal of Clinical Endocrinology and Medatolism. 68. (1989)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi