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Studies on fetal wound healing in a culture model

Research Project

Project/Area Number 63570609
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionKitasato University

Principal Investigator

IHARA Setsunosuke  Kitasato Univ.Sch. of Medicine, Associate Prof., 医学部, 助教授 (90101295)

Co-Investigator(Kenkyū-buntansha) FURUYAMA Nobutaka  Kitasato Univ.Sch. of Medicine, Lecturer, 医学部, 講師 (60165503)
UTSUGI Ryuichi  Kitasato Univ.Sch. of Medicine, Lecturer, 医学部, 講師 (60151908)
UCHINUMA Eiju  Kitasato Univ.Sch. of Medicine, Lecturer, 医学部, 講師 (90146465)
SHIOYA Nobuyuki  Kitasato Univ.Sch. of Medicine, Professor, 医学部, 教授 (80050376)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsWound healing / Fetus / Organ culture / Wound closure / Epidermalization / Rat / ラット胎仔 / フィブリン形成 / in vitroモデル
Research Abstract

1. The in vitro wound healing responses in whole-limb organ culture of fetal rats at day 18 of gestation were compared with the intrauterine wound repair. In vitro, after a skin incision was made in the forelimb, the subcutaneous tissue and the dermis regenerated. Reepithelialization appeared within two to five days. Thus, the wound healing was comparable to the intrauteine fetal wound repair. The organ culture may be a useful and suitable in vitro test system to study the fetal wound healing mechanisms.
2. Forelimb buds of rat embryos at day 12 of gestation were cut and cultured in the same manner as above. In the absence of fetal calf serum or Ultroser G, a serum substitute, epidermal wound closure appeared in a concentration-dependent manner within 1 day of culture. Cycloheximide reversibly inhibited wound closure, showing that protein synthesis was required for epidermal wound closure. HPLC of Ultroser G indicated that high molecular weight factors ( greater than 25 KD ) were required for epidermal wound closure.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Kistler,Andreas: "Wound healing in fetal limb organ culture" Annals of Plastic Surgery. 21. 303-309 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宇津木龍一: "胎児手術による無瘢痕手術の可能性" 北里医学. 19. 85-93 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kistler,Andreas: "Requirement of serum factors for wound closure of embryonic skin in vitro" Annals of Plastic Surgery. 23. 479-487 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kistler, Andreas et al.: "Wound healing in fetal limb organ culture" Ann.Plast.Surg.21, 303-309, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Utsugi, Ryuichi et al.: "Is it possible to perform fetal surgery without leaving a scar?" Kitasato Medicine 19, 85-93, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kistler, Andreas et al.: "Requirement of serum factors for wound closure of embryonic skin in vitro" Ann.Plast.Surg.23, 479-487, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kistler,Andreas: "Requirement of serum factors for wound closure of embryonic skin in vitro" Annals of Plastic Surgery. 23. 479-487 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 猪原節之介: Developmental Biology.

    • Related Report
      1988 Annual Research Report
  • [Publications] 猪原節之介: Developmental Biology.

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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