| Project/Area Number |
63570635
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Tottori University School |
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Principal Investigator |
KIMURA Osamu Tottori University School of Medicine Assistant, 医学部, 助手 (20169923)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Akihiko Tottori University Hospital of Medicine, Assistant, 医学部附属病院, 助手 (80186311)
NISHIDOI Hideaki Tottori University School of Medicine, Assistant, 医学部, 助手 (30172670)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Nuclear DNA Content / Gastric Cancer / Colorectal Cancer / Biological Malignancy / Preoperative Diagnosis / Ploidy Pattern / Flowcytometer / Microspectrophotometer |
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| Research Abstract |
Nuclear DNA content was microspectrophotometrically measured in 151 gastric carcinomas and 23 colorectal carcinomas, to assess the predominance of a particular DNA distribution pattern in carcinoma of the stomach, colon and rectum with biologically high malignancy, such as the marked tendency for severe vessel invasion and remote metastasis, and for early recurrence after curative surgery. On the other hand, nuclear DNA content was flowcytometrically analyzed in 12 gastric carcinomas and 6 colorectal carcinomas both in biopsy specimen from endoscopy and in resected- specimen, to investigate correspondence between them.
The incidence of high DNA ploidy was higher in cases with severe vessel invasion and remote metastasis among cases of the same depth of invasion, and in dead cases of early recurrence among cases of the same histological stage. Furthermore, there was a close correspondence in DNA ploidy pattern between biopsy and resected specimen except one case.
Therefore, we consider that the DNA distribution pattern well reflects the biological malignancy in carcinoma of the stomach, colon and rectum. Further, examination of the DNA ploidy in preoperative biopsy may offer a good clue to the appropriate choice of treatment of carcinoma according as its biological malignancy.
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