Cell Culture and Molecular Biologicals Study for Human Esophageal Carcinom
Grant-in-Aid for General Scientific Research (C)
|Allocation Type||Single-year Grants |
|Research Institution||Yamaguchi University |
MURAKAMI Takuo Yamaguchi University School of Medicine. Second Department of Surgery. Associate Professor, 医学部, 助教授 (10091216)
JOHONO Norifumi Yamaguchi University School of Medicine. Hospita Second department of Surgery., 医学部附属病院, 助手 (70196666)
OKA Masaaki Yamaguchi University School of Medicine. Second department of Surgery., 医学部, 講師 (70144946)
SHIMIZU Ryouichi Yamaguchi University School of Medicine. Hospital. Second department of Surgery., 医学部附属病院, 助手 (30187471)
KUSANAGI Hiroshi Yamaguchi University School of Medicine. Hospital Second department of Surgery.
丹黒 章 山口大学, 医学部, 助手 (10197593)
本間 喜一 山口大学, 医学部附属病院, 助手 (20144933)
|Project Period (FY)
1988 – 1990
Completed (Fiscal Year 1990)
|Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
|Keywords||Esophageal cancer / cell line / Southern blotting / erb-B / myc / EGF / serum-free culture / Etoposide / 食堂癌 / EGF recepter / ヌ-ドマウス / 樹立 / erbB / VPー16 / ヌ-ドマウス皮下移植腫瘍 / in vitro培養系 / Southern blot hybrydazation / 増殖因子 / 増殖因子レセプタ- / 核酸分析 / 腫瘍マーカー / 免疫組織化学 / ヌードマウス移植系 / 培養細胞|
Six squamous cell carcinoma cell lines, YES-1, YES-2, YES-3, YES-4, YES-5 and YES-6, were established from human esophageal carcinoma.
YES-1 and YES-2 were established from subcutaneus small tumor implanted into nude mice, which had been transplanted from a surgical specimen obtained from esophageal cancer patients.
YES-3 was established from pleural effusion of the carcinomatous pleuritis for carcinoma of the esophagus. The remaining YES-4, YES-5 and YES-6 were derived from a surgical specimen.
Deoxyribonucleic acids were extracted from these 6 human esophageal carcinoma cell lines using the phenol-chloroform method. The C-erb B gene and the C-myc gene were examined for possible structural changes in them by Southern blot hybridization. The C-erb B gene was amplificated about ten folds in YES-1 and amplification of C-myc could not be detected in these six cell lines.
In our laboratory, we have a problem concerning the manipulation of Southern blot hybridization, and we must check the resu
The amplification of various oncogenes will be examined by using Northern blotting method, when the manipulation procedure has been mastered.
We are examining the role of EGF in vitro and in vivo using YES-2, which had the shortest dubbing time among the six cell lines. We are going to examine it using five other cell lines in near future. Additionally we will determine the binding of EGF to membrane fractions.
YES-1 and YES-2 were adapted to the serum-free medium. These two lines may produce some substances, such as growth factors.
We are now examining four other cell lines which have been adapted to the serum-free medium.
The influence on cell proliferation of etoposide which is an anticancer drug was examined in YES-1 cells using flow cytometric analysis.
Furthermore, we have scheduled to examine various kinds anticancer drugs, such as cisdiamine dichloroplatinum, vindesin, adriamycin and 5-fluorouracil sensitivity by using these 6 cell lines.
These 6 cell lines may provide useful models for the study of human esophageal carcinoma. Less
Report (4 results)
Research Products (4 results)