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Simultaneous measurement of tissue oxygen concentration and monoamine metabolites in ischemic brain

Research Project

Project/Area Number 63570678
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionNagoya University

Principal Investigator

SHIBUYA Masao  Nagoya University Associate Professor School of Medicine, 医学部, 助教授 (10135345)

Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1988: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCerebral ischemic / Neurotransmitter / Monoamine / In vivo voltammetry / Oxygen concentration / 脳循環
Research Abstract

Using in vivo voltammetry tissue oxygen concentration and monoamine neurotransmitter metabolism were measure in ischemic brain in rats.
From previous experiments it was known that oxygen concentration could be measured with a voltage of -400mV while that of Dopamine/Dopac and 5-HIAA at +150mV and +300mV respectively. Carbon fibermicroelectrode was inserted into to caudate nucleus of rats. Tissue oxygen tension thus measured correlated well with that of condition of respiration : room air, pure oxygen, choking. The electrode was still usable 3 months after implantation.
As a model of complete brain ischemia KCl was injected intravenously. Striatal DA/DOPAC quickly rose reaching 900% of the control at 10 min, while 5-HIAA rose to 300%, then both gvadually decrease. Similar results were obtained in ischemic rat model of Pulsinelli.
From above results it was shown that in vivo voltammetry could be used to measure both tissue oxygen tension and monoamine metabolism in ischemic rat brain. However, these values are modified depending upon the change in tissue pH, temperature which varies during ischemia and exact measurement of these parameters is essential to correct obtained values. Further experiments are needed in that field. It was considered that this method could be used for evaluation of effects of drug treatment of ischemia in live animals.

Report

(4 results)
  • 1991 Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Koichiro Ogura Masato Shibuya et al.: "Changes in striatal dopamine metabolism measured by in vitro voltammetry during ischemia in rats." Stroke. 20. 783-787 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toshio Asano,Masato Shibuya,et al.: "Endothelin and the production of cerebral vasospasm in dogs." Biochem Biophys Res Commun. 159. 1345-1357 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Shibuya,Y.Suzuki et al.,: "Effects of STA-MCA anastomosis for ischemic oculopathy due to occlusion of the internal carotid artery." Acta Neurochirur. 103. 71-75 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Shibuya,Y.Suzuki et al.: "Dose escalation trial of a novel calcium antagonist 'AT877' in patients with subarachnoid hemorrhage." Acta Neurochirur. 107. 11-15 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sh.Satoh,M.Shibuya,et al.: "The effects of HA1077 on the cerebral circulaiton after subarachnoid hemorrhage in dogs." Acta Neurochirur. 110. 185-188 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koichiro Ogura, Masato Shibuya et al.: "Changes in striatal Dopamine metabolism measured by in vivo voltammetry during ischemia in rats." Stroke. 20. 783-787 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toshio Asano, Masato Shibuya et al.: "Endothelin and the production of cerebral vasospasm in dogs." Biochem Biophys Res Commun. 159. 1345-1357 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Shibuya, Y. Suzuki et al.: "Effects of STA MCA anastomosis for ischemic oculopathy due to occlusion of the internal carotid artery." Acta Neurochirur. 103. 71-75 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Shibuya, Y. Suzuki et al.: "Dose escalation trial of a novel calcium antagonist 'AT877' in patients with subarachnoid hemorrhage." Acta Neurochirur. 107. 11-15 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sh. Satoh, M. Shibuya et al.: "The effects of HA1077 on the cerebral circulation after subarachnoid hemorrhage in dogs." Acta Neurochirur. 110. 185-187 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Shibuya;Y.Suzuki;M.Takayasu;T.Asano;T.Harada;I.Ikegaki;S.Satoh: Acta Neurochirurg.90. 53-59 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Okada;M.Shibuya;Y.Suzuki;I.Ikegaki;N.Kageyama;T.Asano;H.Hidaka: Neurol.Med.Chir.28. 625-630 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Shibuya;Y.Suzuki;M.Takayasu;T.Asano;T.Harada;I.Ikegaki;S.Satoh: "The effects of the calcium antagonist HA1077 on experimental vasospasm" Cerebral Vasaspasm R.H.Wilkins(ed)Raven Press,New York, 457-461 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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