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Ischemic Neuronal Damage and Excitatory Amino Acid Neurotransmitter

Research Project

Project/Area Number 63570687
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionTeikyo University School of Medicine

Principal Investigator

KIRINO Takaki  Teikyo Univ. School of Med., Associate Prof., 医学部, 助教授 (10161605)

Co-Investigator(Kenkyū-buntansha) TAKAGI Kiyoshi  Teikyo Univ. School of Med., Assistant Prof., 医学部, 講師 (40197059)
GOTOH Osamu  Teikyo Univ. School of Med., Assistant Prof., 医学部, 講師 (40134598)
TAMURA Akira  Teikyo Univ. School of Med., Professor, 医学部, 教授 (80111532)
TAKUSAGAWA Yutaka  Teikyo univ. School of Med., Assistant Pro (10179591)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
KeywordsCerebellum / Purkinje's cell / Granule cell / Ischemia / Cell death / Neurotransmitter / Glutamate / ischemia / cerebllum / intracranial pressure / Purkinje cell / cell death / プルキン工細胞 / 顕粒細胞
Research Abstract

Transient global ischemia was induced for 10 min in the rat by raising the intracranial pressure higher than the systemic arterial blood pressure. The animals were perfusion-fixed at 3h, 6h, 12h, 1d, 2d, or 4d following the ischemic insult. Ischemic neuronal alteration was always found in the cerebellum throughout the observation period. The change in the hippocampus was only seen in the specimen obtained 4d after ischemia. Three hours following ischmia, Purkinje's cells looked darkly stained to basic dyes such as toluidine blue and hematoxylin. Six hours after ischemia, darkly Stained Purkinje's cells were more than half of the whole Purkinje's cell population. Dark staining was observed throughout the Purkinje's cell including cell body, nucleus, and dendrite. The inside structure of the dark Purkinje's cell was well maintained and there was no empty swelling or condensation of the cell organelles. Twelve hours after ischemia and later, darkly stained cell disappeared and whole Purkinje's cell number remained unchanged. Two days following ischemia, 25% decrease of Purkinje's cell number was seen. In hematoxylin-eosin stained specimen at this stage, acidophilic neurons were scattered in the Purkinje's cell layer. In the granule cell layer, swollen Purkinje's cell axon was seen from 3h through 4d specimens. The results indicate that dark cell change is, at least partially, a reversible type of ischemic alteration. Following ischemia, unlike hippocampus, Purkinje's axon is involved in the development of ischemic neuronal damage. There seem to be difference in the distribution of excitatory neurotransmitter receptors between the hippocampus and cerebellum. The morphological difference found here may be a reflection of the diversity in receptor subtype distribution.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yamamoto M.,Tamura A.,Kirino T.,Shimizu M.,Sano K.: "Behavioral changes after focal cerebrql ischemia by left middle cerebral artery occlusion in rats" Brain Res. 452. 323-238 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki T.,Nakagomi T.,Kirino T.,Tamura A.,Noguchi M.,Saito I.,Takakura K.: "Indomethacin ameliorates ischemic neuronal damage in the gerbil hippocampal CA1 sector" Stroke. 19. 1399-1403 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤江 和貴,桐野 高明,田村 晃,岡 秀宗,他: "局所脳虚血後にみられた視床の変性・萎縮" 医学のあゆみ. 148. 621-622 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kirino T.,Tamura A.,Sano K.: "Chronic maintenance of presynaptic terminal in gliotic hippocampus following ischemia" Brain Res. 510. 17-25 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujie W.,Kirino T.,Tomukai N.,Iwasawa T.,Tamura A.: "Progressive shrinkage of the thalamus following middle cerebral artery occlusion in rats" Stroke. 21. 1485-1488 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kirino T.,Tsujita Y.,Tamura A.: "Induced to tolerance to ischemia in gerbil hippocampal neurons" J Cereb Blood Flow and Metab.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kirino T, Tamura A, Sano K.: "Chronic maintenance of presynaptic terminals in gliotic hippocampus following ischemia." Brain Res. 510. 17-25 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujie W, Kirino T, Tomukai N, Iwasawa T, Tamura A.: "Progressive shrinkage of the thalamus following middle cerebral artery occlusion in the rat --long-term effect of focal cerebral ischemia--" Stroke. 21. 1485-1488 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kirino T, Tsujita Y, Tamura A: "Induced tolerance to ischemia in gerbil hippocampal neurons." J Cereb Blood Flow and Metab.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kirino T,Tamura A,Sano K:"Chronic maintenance of presynaptic terminal in gliotic hippocampus following ischemia" Brain Res. 510. 17-25 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tamura A,Kirino T,Sano K,Takagi K, Oka H:"Atrophy of the ipsilateral substantia nigra following middle cerebral artery occluison in the rat" Brain Res. 510. 154-157 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田村 晃、桐野 高明、藤江 和貴、後藤 修、他: "中大脳動脈領域の梗塞に伴う同側視床の萎縮" 医学のあゆみ. 152. 619-620 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujie W,Kirino T,Tomukai N,Iwasawa T,Tamura A:"Progressive shrinkage of the thalamus following middle cerebral artery occlusion in rats." Stroke. 21. 1485-1488 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kirino T,Tsujita Y Tamura A:"Induced to tolerance to ischemia in gerbil hippocampal neurons" J Cereb Blood Flow and Metab.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kirino T,Tamura A,Sano K.: "Chronic maintenance of presynaptic terminals in gliotic hippocampus following ischemia." Brain Res.

    • Related Report
      1989 Annual Research Report
  • [Publications] Fujie W,Kirino T,Tomukai N,Iwasawa T,Tamura A.: "Progressive shrinkage of the thalamus following middle cerebral artery occlusion in the rat--Long-term effect of focal cerebral ischemia--" Submitted to Stroke.

    • Related Report
      1989 Annual Research Report
  • [Publications] Kirino T,Nakagomi T,Tomukai N,Tamura A.: "Transient dark cell change in rat cerebellar purkinue cells following compression ischemia."

    • Related Report
      1989 Annual Research Report
  • [Publications] Kirino,T,;Tamura A,;Sano K:"Early and late neuronal damage following cerebral ischemia." Plenum Publishing Corporation, 23-34 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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