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Ultrastructural and Histological Studies on Aging of Joint Tissues

Research Project

Project/Area Number 63570704
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionNagoya City University Medical School

Principal Investigator

KANIE Ryoichi  Nagoya City Univ. Medical School, Assoc. Prof., 医学部, 助教授 (80080000)

Co-Investigator(Kenkyū-buntansha) IMAIZUMI Tsukasa  Nagoya City Univ. Medical School, Lecturer, 医学部, 講師(非常勤) (70080033)
TAKASHIMA Masahumi  Nagoya City Univ. Medical School, Researcher
INAMOTO Masahiro  Nagoya City Univ. Medical School, Researcher
高嶋 雅文  名古屋市立大学, 医学部, 研究員
稲本 正博  名古屋市立大学, 医学部, 研究員
脇田 郷  名古屋市立大学, 医学部, 研究員
杉本 勝正  名古屋市立大学, 医学部, 助手 (10206427)
鈴木 洋  名古屋市立大学, 医学部, 研究員
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsArticular Cartilage / Aging of Cartilage / Degeneration of Cartilage / Repair of Cartilage / Electron Microscopic Findings / Degenerative Substances / Cell Debris / Metabolism of Chondrocyte / 軟骨細胞内変性物質 / 軟骨細胞集団 / 軟骨膠原原線維 / 関節軟骨の変性・老化 / ミエリン様物質 / 変性ミトコンドリア / 関節軟骨の老化 / 変形性関節症軟骨所見 / 破壊軟骨細胞破片
Research Abstract

(1) From our studies, it becomes clear that on the articular cartilage especially aging related, findings appear from a relatively early age, compared with other articular tissues. (2) In the cytoplasm of many chondrocytes of old rats, nubecular substances are observed. They lie scattered or come together in small or large masses, and sometimes large parts of the chondrocyte are occupied and filled by such substances, which are considered to be degenerative substances. (3) In aged chondrocytes, cytoplasmic organelles decrease or disappear, cytoplasmic density goes down, and sometimes many microtubules are well observed. Further consideration is needed as to whether these microtubules increase as one of the aging phenomena or they exist from the beginning as cell skeletons which simply become more visible as a result of aging changes in the cell. (4) In the extracellular matrix, granular and dot-like substances are scattered, and some such substances are enveloped by a membrane, like a … More lisosome. These outside substances may be considered to be cell debris originated from other destroyed cells and to work enzymatically to damage the intercellular matrix. (5) In the chondrocytes of osteoarthritic cartilage, many and various cell-inclusions, considered to show metabolic abnormality, appear, increase and occupy large parts of the cytoplasm. The appearance of lipid droplets is notable, and in high degrees of degeneration, their size and number increase. Many intracytoplasmic fine flaments lie scattered in a bundle. Lighter stained, degenerated glycogen granules come together in large masses. (6) In highly degenerated or destroyed cytoplasm, small or large masses of nubecular substances, considered to be degenerative substances, occupy large parts of the cytoplasm. This finding resembles that observed in old rats. (7) Many mitochondria show deformity and some of them are destroyed. It is an interesting finding that myeloid bodies with a lamellar structure are often observed in the cytoplasm and seem to be associated with the mitochondria. Its existence suggests cytoplasmic degeneration. (8) Deformed chondrocytes, whose interiors are fibroblast-like, are also observed, surrounded by a lot of bundled collagen fibrils. It may suggest repair of the cartilage. (9) The punched wound, which reached to the subchondral bone, is ffilled from the bottom. Fibrous connective tissue grows to mature by and by, and expands around and over the wound. (10) These findings suggest that the repair of hyaline cartilage will be made by the granulation and fibrous cartilage from subchondral bone itself. itself. Less

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ryoichi KANIE: "Ultrastructural Study on the Degeneration of Aged Articular Cartilage" J. Clim. Electron Microscopy. 21. 639-640 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryoichi KANIE et al.: "Degeneration of Articular Cartilage, with Particular Reference to the Electron Microscopic Findings of Osteoarthritis" J. Clim. Electron Microscopy. 22. 560-561 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 蟹江 良一: "軟骨の超微形態と修復像 ー透過型電顕所見を中心にー" 日本整形外科学会雑誌. 64. S892 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 蟹江 良一: "人体組織学(分担執筆) 第1巻、概説・運動器、II運動器、1.軟骨組織" 朝倉書店(東京), 159-184 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanie, R., Yamada, K. and Inamoto, M.: "Ultrastructural study on the degeneration of aged articular cartilage" J. Clin. Electron Microscopy. 21. 639-640 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanie, R., Yamada, K. and Shimizu, H.: "Degeneration of articular cartilage, with particular reference to the electron microscopic findings of osteoarthritis" J. Clin. Electron Microscopy. 22. 560-561 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanie, R.: "Ultrastructure and restoration of articular cartilage, focusing on transmission electron microscopic findings." J. Jpn. Orthop. Assoc.64. 892 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanie, R.: "Articular Cartilage" "Histology of Human Body". Asakura-Shoten (Tokyo). 159-184 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ryoichi KANIE,et al.: "Degeneration of Articular Cartilage, with Particular Reference to the Electron Microscopic Findings of Osteoarthritis" J. Clim. Electron. Microscopy. 22. 560-561 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 蟹江 良一: "軟骨の超微形態と修復像 ー透過型電顕所見を中心にー" 日本整形外科学会雑誌. 64. S892 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ryoichi KANIE et al.: "Ultrastructural study on the degeneration of aged articular cartilage" J.Clim.Electron Mecroscopy. 21. 639-640 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ryoichi KANIE et al.: "Depeneration of Articular Cartilage,with Particular Reference to the Electron Microscopic Findings of Osteoarthritis" J.Clim.Electron Mecroscopy. 22. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ryoichir KANIE,et al.: J.Clin.Electron Microscopy. 21. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 蟹江良一: "人体組織学第1巻.概説・運動器,II運動器,1.軟骨組織" 朝倉書店, 159-184 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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