| Project/Area Number |
63570711
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The Jikei University, School of medicine |
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Principal Investigator |
ASANUMA Kazuo The Jikei University Orthopaedic surgery Lecturer, 医学部・整形外科, 講師 (50159373)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIKAWA Ko The Jikei University Orthopaedic surgery Assistant, 医学部・整形外科, 助手 (80209030)
IIZUKA Hisaharu The Jikei University Orthopaedic surgery Assistant, 医学部・整形外科, 助手 (60168060)
SATOMURA Toshiaki The Jikei University Orthopaedic surgery Assistant, 医学部・整形外科, 助手 (10154121)
SAITO Kiyoto The Jikei University Orthopaedic surgery Assistant, 医学部・整形外科, 助手 (40162193)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Hamster osteosarcoma / 111In-labeled Monoclonlantibody / Radioimmunodetection / 骨腫瘍 / 肺転移 / モノクローナル抗体 / イメージング |
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| Research Abstract |
An experiment hamster osteosarcoma was induced by BK virus (Uchida S, et. al.), and the usefulness of this tumor was confirmed as an experimental model of the diagnosis and treatment of osteosarcoma. All the hamsters with this tumor died of spontaneous lung metastasis within one month. Monoclonal antibodies against hamster osteosarcoma Os5l5 were obtained by the production and cloning of hybrids resulting from the fusion of the mouse myeloma cells (P3U1) with spleen cells from Os5l5-immunized BALB/ c mice. Four hybrid clones were established and designated as Nos. 10, 15, 22 and 29. In imunohistochemistry (ABC), antibody Nos. 10, 15 and 22 react with tumor tissue matrix, while No. 29 reacts with a cell surface antigen. We used monoclonal antibody No. 29 because it reacts specifically with tumor cells, and does not react with normal organ cells.
Monoclonal antibody No. 29 (mouse immunoglobulin G1) against Os5l5 were isolated from ascitic fluid and labeled with ^<111>In. After injection to hamsters carrying Os5l5, the uptake of radioactivity in the tumor, visceral organs and blood was determined. Three days after injection, preferential accumulation was seen in the tumors (tumor to blood ratio: 4.0). Furthermore, in hamsters with renal cancer (THK) unreactive with No.29, there was no significant localization of radiolabeled No. 29 in the tumor.
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