| Project/Area Number |
63570713
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
ABE Muneaki Osaka Medical College, Faculty of Medicine, Associate Professor, 医学部, 助教授 (70084994)
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| Co-Investigator(Kenkyū-buntansha) |
DOI Munekazu Osaka Medical College, Faculty of Medicine, Research Associate., 医学部, 助手 (70197995)
KINOSHITA Mituo Osaka Medical College, Faculty of Medicine, Lecturer., 医学部, 講師 (90169912)
土肥 恒夫 大阪医科大学, 医学部, 助手 (70188853)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | cytofluorometer / nerve regeneration / Ribonucleic acid / spinal ganglion |
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| Research Abstract |
The activity of the nerve cell body has been regarded as a factor that greatly influences axon regeneration. We have devised a method to measure the amount of ribonucleic acid in nerve cells using reflected fluorescence microscopy. Using this method, quantitative changes in the ribonucleic acid in spinal ganglion cells after nerve division were observed. Additionally, the influence of stump neuroma amputation on these quantitative changes in ribonucleic acid content was studied and the significance of neuroma amputation at the time of nerve suture was emphasized. Results are as follows ; 1. It was found that using a section having a thickness of 6mu m made it possible to carry out a highly accurate comparative quantity of ribonucleic acid content in the nerve cells.
2. The amount of ribouncleic acid in the nerve cells and the area of the cross section of nerve cells increased gradually after sciatic nerve division, and the values reached a maximum one week after nerve division.
3. The amount of ribonucleic acid in the nerve cell body increased significantly after neuroma amputation
4. Even though the time of the neuroma resection was changed, no increase in the rebonucleic acid content greater than the maximum value reached following the initial nerve division was observed in our studies.
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