| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
Antifertility effect of Sulfasalazine(SASP) has been reported by many authors, but its mechanism has not been clear, and the target organ has not been detected. Our purpose of this experiment was to study the antifertility effect of both metabolites of SASP(Sulfapyridine and 5-Aminosalicylate) in male rats and to detect the target organ of Sulfapyridine.
Nine-week-old male Sprague Dawly rats, assigned at random to 12 groups, were treated with Sulfapyridine(SP) at 250, 125, 62.5, 31.3mg/kg. body wt/day, and 5-Aminosalicylate(5-ASA) at 153, 76.5, 38.3, 19.Omg/kg. body wt/day and distilled water, for a period of 5 weeks in addition to a normal diet. Mean fertility, testicular weight and diameter ot the seminal tubules in each group was compared. There was a dramatical suppression in fertility in the groups with SP 250mg/kg. day and 125mg/kg. day, however, no suppression was observed in the other groups. No suppression was observed in the mean testicular weight and the mean diameter of the seminal tubules in all groups.
The concentration of Acetyl-sulfapyridiie(Ac-SP) in the testis, epididymal head and epididymal tail of the rats treated with SP(250mg/kg. for 5 weeks), were investigated by high pressure liquid chromatography.
The concentration of AC-SP was confirmed to be highest in the epididymal head and lowest in the testis. These findings strongly support the hypothesis that SP is the metabolite of SASP, suppressing male fertility and that main target organ of SP is epididymis.
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