| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
It is considered that the anticancer functions of vitamin E include its antiosidant properties, its function as a free radical scavenger, and its immunomodulating functions. with thes considerations in mind, we studied the chemopreventive effect of vitamin E on rat's with bladder tumors induced by N-butyl-N-(4-hydroxybutyl) nitrosamin (BBN). Three-week-old male Wister rats were fed by a diet containing 0(A), 2mg(B), 40mg(C), and 60mg(D) of vitamin E per 100g of food, respectively, by 30 weeks old. Meanwhile, the carcinogen, 0.05%BBN in drinking water were given from 9 weeks old for 8 weeks, and all rats were killed at 30 weeks old.
The number and the size of tumor, the thickness of epithelial cell layers in hyperplasia and the diameter of papillary tumor, and the cellular atypia were assessed by using a point method according to U-test. As assessment of immune response, the lymphocyte count in blood, and CD_4 (helper/inducer-T cell)/CD_8 (suppressor/cytotoxic-T cell) ratio in blood by f
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low cytometry using monoclonal antibodies of them were evaluated according to t-test. Moreover, the correlation between CD/CD, cellular atypia, and serum level of vitamin E were evaluated by coefficient of correlation.
The results obtained are as follow that there was no difference between each group about the number and the size of tumor, the cellularity in A group was significantly more than in D group (P<0.05), the point cellular atypia tended to decrease, with increasing doses of the vitamin E, but not significantly, whereas the CD_4/CD_8 tended to increase by increasing the doses of vitamin E, which showed hignly inverse coefficient of correlation (r = -0.9).
In conclusion, if the correlation between the cellular atypia, the CD_4/CD_8, and the dose of vitamin E are considered, this study suggested vitamin E might have on inducing antigenicity of tumor cell according to dose. However, as there was no clear favorable effect of vitamin E against tumorigenicity, to advance the chemopreventive study by vitamin E should be justified with more meticulous schedule. Less
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