|Budget Amount *help
¥2,400,000 (Direct Cost : ¥2,400,000)
Fiscal Year 1990 : ¥300,000 (Direct Cost : ¥300,000)
Fiscal Year 1989 : ¥300,000 (Direct Cost : ¥300,000)
Fiscal Year 1988 : ¥1,800,000 (Direct Cost : ¥1,800,000)
Studies were undertaken to examine the chemosensitivity of ovarian clear cell carcinoma in vitro by MTT assay.
Four kinds of established human ovarian clear cell carcinoma cell line, HAC-2, OVAS, RMG-I and KK were used in this study.
Twenty-three kinds of antitumor agents including ADM, THP-ADM, EPI, ACR, DM, PEP, BLM, Act-D, MMC, NCS, VLB, VCR, VDS, CPM, MTX, Ara-C, 5-FU, L-ASP, CDDP, 254-S, CBDCA, NCNU, ETP were examined.
The cells were inoculated into microplate at 1 or 3x10^4/well (day 0), cultured for 3 days and exposed to each drug at 0, 0.01, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 mic g/ml, for 1, 3, 6, 12, 24, 48, 72hs (day 3), washed free drug and incubate media alone. The enzyme activities were measured by MTT assay (day 7). The antitumor effects of each drug were evaluated from the surviving fraction curves.
As a result of this study, RMG-I had multidrug resistance and none of drugs were effective. Other 3 kinds of cell lines have similar drug sensitivities and the most effective drugs on this tumor were Act-D, CDDP and 254-S.