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Physiological roles of growth factors in mammalian reproduction.

Research Project

Project/Area Number 63570771
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionThe University of Tokyo

Principal Investigator

TSUTSUMI Osamu  University of Tokyo, Department of Obstetrics and Gynecology, Assistant, 医学部・附属病院・産婦人科, 助手 (60134574)

Co-Investigator(Kenkyū-buntansha) AYABE Takuya  University of Tokyo, Department of Obstetrics and Gynecology, Assistant, 医学部附属病院産婦人科, 助手 (50184247)
YANO Tetsu  University of Tokyo, Department of Obstetrics and Gynecology, Assistant, 医学部附属病院産婦人科, 助手 (50182390)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥800,000 (Direct Cost: ¥800,000)
KeywordsGrowth factor / Epidermal growth factor / EGF / Gamete / Sperm / Oocyte / Embryo / Fetus / EGF / Epidermal growth factor(EGF) / 卵 / 子宮 / 流産
Research Abstract

Physiological roles of growth factors especially epidermal growth factor (EGF) were studied in the field of mammalian reproduction and major findings are as follows :
I. Establishment of pregnancy and fetal growth.
Plasma EGF concentrations were elevated during pregnancy in human and there was positive correlation between maternal and fetal EGF levels although fetal levels were significantly lower than maternal ones.
Sialoadenectomy (removal of the submandibular glands) of pregnant mice was shown to cause EGF deficiency during pregnancy which resulted in intrauterine growth retardation (IUGR). This makes an animal model to study the mechanism of fetal growth and IUGR.
II. Maturation of oocyte and spermatozoa.
EGFlike substance (EGF-L) was present in the human follicular fluid obtained from patients in our in vitro fertilization program. The concentration of EGF-L was related with the maturity of oocyte within. EGF-L was also present in human seminal fluid and concerned with sperm morbidity.
EGF stimulated the maturation of rat oocyte in culture in terms of both germinal vesicle breakdown (resumption of meiosis) and phosphofructokinase activity (rate-limiting enzyme present in the cytoplasma).
III. Development of preimplantation embryo.
Presence and number of EGF receptor was determined using binding assay with I-125 EGF. Presence of EGF receptor was demonstrated in mouse oocyte and the number of EGF receptor was increased as embryos developed in preimplantation period. EGF added to the culture medium stimulated the development of 2-cell embryos.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] 堤 治: "配偶子の成熟とepidermal growth factor(EGF)" 日本受精着床学会雑誌. 6. 19-21 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 堤 治: "Epidermal growth factor(EGF)と妊娠・胎児発育" 臨床婦人科産科. 43. 247-251 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 堤 治: "卵胞の発育成熟とepjidermal growth factor(EGF)" 産婦人科の世界. 41. 367-373 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masahiko Mizuno: "Role of prostaglandins in egg implantation." Advances in gynecological endocrinology Parthenon Publishing Group. 1. 433-439 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Osamu Tsutsumi: "Studies of hexokinase activity in human and mouse oocyte." American Journal of Obstetrics and Gynecology. 62. 1301-1304 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 堤 治: "卵成熟機構の生化学的解析" 日本受精着床学会雑誌. 8. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Tsutsumi, T. Ayabe, T. Yano, K. Satoh and M. Mizuno: "Production of progesterone by preimplantation embryo : a possible factor controlling early embryo development." Human Reproduction. 509 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Tsutsumi: "A physiological function of epidermal growth factor in spermatogenesis and oocyte maturation." Japan Journal of Fertilization and Implantation. 6. 19 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ayabe, O. Tsutsumi, T. Yano, N. Mitsuhasi and M. Mizuno: "Presence of steroid metabolizing activity in mouse preimplantation embryo." Japan Journal of Fertilization and Implantation. 6. 30 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Fujiwara, O. Tsutsumi, T. Ayabe, T. Yano, N. Mitsuhasi and M. Mizuno: "Protein synthesis of pre- and post-fertilization mouse oocyte." Journal of Mammalian Ova Research. 6. 43 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Mizuno, N. Mitsuhasi, O. Tsutsumi, O. Ishihara, K. Kinoshita and K. Satoh: "Role of prostaglandins in egg implantation." Advances in Gynecological Endocrinology. 1. 433 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ayabe, O. Tsutsumi, T. Yano, T. Fujiwara, N. Mitsuhasi and M. Mizuno: "Possible role of progesterone on the activation of protein and RNA synthesis of preimplantation mouse embryo." Journal of Mammalian Ova Research.6. 45 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Tsutsumi T. Yano, K. Satoh, M. Mizuno and T. Kato: "Studies of hexokinase activity in human and mouse oocyte." American Journal of Obstetrics and Gynecology. 162. 1301 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Fujiwara, O. Tsutsumi, T. Ayabe, T. Yano, N. Mitsuhasi, M. Mizuno and S. Miyazaki: "Electrophysiological study on the maturation of hamster oocytes." Journal of Mammalian Ova Research. 7. 29 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. M. Kuo, Y. Taketani, T. Ayabe, H. Gomibuchi, T. Yano, O. Tsutsumi and M. Mizuno: "Influence of peritoneal fluid width endometriosis on the development of mouse embryos." Japan Journal of Obstetrics and Gynecology. 42. 1284 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] O. Tsutsumi, T. Yano, T. Ayabe, T. Fujiwara and I. Hosoya: "Biochemical analysis of oocyte maturation." Japan Journal of Fertilization and Implantation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Osamu Tsutsumi: "Studies of hexokinase activity in human and mouse oocyte." American Journal of Obstetrics and Gynecolgy. 162. 1301-1304 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 堤 治: "卵成熟機構の生化学的解析" 日本受精着床学会雑誌. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 堤 治: "配偶子形成調節機構の分子細胞生物学的解析" 日本医学会誌. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤原 敏博: "ハムスタ-卵成熟過程の電気生理学的解析" 哺乳動物卵子研究会誌. 7. 29-30 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 加藤 尚彦: "マウス未受精卵および受精卵の成熟過程:単一卵のエネルギ-代謝の分析" 「生殖系列」ニュ-スレタ-. 6. 52-60 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 郭 宗明: "子宮内膜症腹腔内貯留液のマウス胚発生に及ぼす影響" 日本産科婦人科学会雑誌. 42. 1284-1290 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 堤 治: "プリンシプル産科学" メジカルビュ-社, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 堤治: "配偶子の成熟とepidermal growth factor(EGF)" 日本受精着床学会雑誌. 6. 19-21 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 堤治: "Epidermal growth factor(EGF)と妊娠・胎児発育" 臨床婦人科産科. 43. 247-251 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 堤治: "Epidermal growth factor(EGF)と性機能" ホルモンと臨床. 37. 205-209 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 堤治: "卵胞の発育成熟とepidermal growth factor(EGF)" 産婦人科の世界. 41. 367-373 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masahiko Mizuno: "Role of prostaglandins in egg implantation." Advances in gynecological endocrinology Parthenon Publishing Group. 1. 433-439 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Osamu Tsutsumi: "Studies of hexokinase activity in human and mouse oocyte." American Journal of Obstetrics and Gynecology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 堤治,水野正彦,岡孝己: 臨床婦人科産科. 42. 303-311 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 堤治: 日本受精着床学会雑誌. 6. 19-21 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 堤治,水野正彦,岡孝己: ホルモンと臨床. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 堤治,亀井良政,桑原廣紀,水野正彦: 臨床婦人科産科. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 綾部琢哉,堤治,矢野哲,三橋直樹,水野正彦: 日本受精着床学会雑誌. 6. 30-31 (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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