| Project/Area Number |
63570776
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Mie University |
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Principal Investigator |
YAMAMOTO Toshihiko Mie University Sch. of med. Assistant Professor, 医学部, 講師 (80135459)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAYA Ken Mie University Sch. of Med. Instructor, 医学部, 助手 (60206399)
NISHIYAMA Masato Mie University Sch. of Med. Instructor, 医学部附属病院, 助手 (10218224)
SUGIYAMA Youichi Mie University Sch. of Med. Professor, 医学部, 教授 (30093131)
山脇 孝晴 三重大学, 医学部附属病院, 助手 (50200853)
野村 浩史 三重大学, 医学部, 助手 (00192261)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Endometrium / Decidualization / Decidual Cell / Epidermal growth Factor / Prolactin / Tyrosine Kinase / Protein phosphorylation / プロティンキナ-ゼC / 細胞成長因子 / Cキナーゼ / プロゲステロン |
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| Research Abstract |
The results were as follows ; (1) Immunohistochemical localization of epidermal growth factor (EGF) were observed in decidualized human endometrium. (2) Production of EGF by decidual cells was clearly demonstrated immunoprecipitation-autoradiography. (3) Scatchard plots revealed decidual cell possesses single kind of EGF receptor with Kd of 1.02 nM and Bmax of 89.4 pM/2x10^5 cells. (4) EGF dosedependently inhibited prolactin production by decidual cells with IC_<50> of 700 pg/ml. (5) The inhibitory effect of EGF on prolactin production was induced by the tyrosine kinase and protein kinase C. (6) EGF enhanced specific protein phoshorylation (48kDa, acidic protein) by the activation of protein kinase C.
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