| Project/Area Number |
63570803
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hokkaido University |
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Principal Investigator |
FUKUDA Satoshi Hokkaido University School of Medicine Lecturer, 医学部附属病院, 講師 (20125347)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA K. Hokkaido University School of Medicine Instractor, 医学部, 助手 (80212835)
SATOH N. Hokkaido University School of Medicine Instractor, 医学部, 助手 (70201530)
TANAKA K. Hokkaido University School of Medicine Associated Professor Chief of Otology of, 助教授 主任医長 (10004946)
間口 四郎 北海道大学, 医学部, 助手 (20199665)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1988: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Guinea Pig Cytomegalovirus (GPCMV) / Endolymphatic sac / Spiral modiolar vein / Inner ear immune response / Viral infection of the inner ear / Guinea Pig Cytomegalovirus(GPCMV) / Guinea Pig Specific Cytomegalovirus(GPCMV) / Action Potential(AP) |
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| Research Abstract |
The endolymphatic sac and cochlea including spiral modiolar vein were investigated in the guinea pig inoculated Guinea Pig Cytomegalovirus (GPCMV) and psoralen-ultraviolet inactivated GPCMV into the cochlea of seronegative and seropositive animal.
Histopathological including immunoelectron microscopic study and electophysiological investigation were carried out at day 2, 4, 6, 8, to elucidate the possible immunological role of endolymphatic sac and cochlea in inner ear viral infection.
The summary of results are as follows ;
1) Hearing loss was found in the seronegative group whereas hearing was protected in the seropositive and control group.
2) It was recognized that inflammatory cells infiltration via collecting venule and spiral modiolar vein from systemic circulation actually occurs in the GPCMV inoculation to the cochlea of seronegative group.
3) No inflammatory cells infiltration was recognized in the cochlea including spiral modiolar vein of seropositive and control group.
4) Only slight inflammatory cells infiltration was found in the endolymphatic sac in this system. But endolymphatic sac reacts immunologically with direct inoculation of GPCMV into the endolymphatic sac.
5) There must be 2 system to react to the stimulation of virus inoculation to the cochlea. Namely, spiral modiolar vein of the cochlea and endolymphatic sac. The degree how these area reacts to the viral infection in the inner ear is still controversial and wait for further precise time cours e study in this particular area.
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