| Project/Area Number |
63570806
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
TAKIMOTO Toru Kanazawa University, School of Medicine, Assistant Professor, 医学部附属病院, 講師 (60143905)
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| Co-Investigator(Kenkyū-buntansha) |
UMEDA Ryozo Kanazawa University, School of Medicine, Professor, 医学部, 教授 (60019528)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | EPSTEIN-BARR VIRUS / NASOPHARYNGEAL CARCINOMA / TUMORIGENICITY / HYBRID CELLS / CLONING / EBウィルス / ヌ-ドマウス / 放射線 / DNA / ヌードマウス |
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| Research Abstract |
We have succeeded in establishing an Epstein-Barr virus (EBV) genome- positive and epithelial cell line by the fusion of EBV-transformed lymphoid cells with an epithelial cells. However, the epithelial hybrid cells (A2L/AH) were nontumorigenic in nude mice during early passage levels. Thereafter, an EBV genome-negative clone and two EBV genome-positive clones of the A2L/AH cells have been successfully cloned. We have continued to culture these three clones, as it is possible that EBV may disturb mechanisms that regulate tumorigenicity when cells are grown in vitro for long periods of time. Two EBV genome-positive A2L/AH clones have become tumorigenic in nude mice after long period of time. The data suggest that EBV may be associated with tumorigenicity in nude mice, at least in part.
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