Experimental Study on Changes of Intraocular Pressure by the Application of Griseolic Acid
| Project/Area Number |
63570832
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MISHIMA Hiromu Hiroshima Univ. School of Med. Associate Professor, 医学部, 助教授 (20034100)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Sigeaki Hiroshima Univ. School of Med. Assistant Prof., 医学部附属病院, 助手 (30196480)
NAKANO Kensuke Hiroshima Univ. School of Med. Assistant Prof., 医学部附属病院, 助手 (30164247)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | griseolic acid / cAMP-phosphodiesterase inhibitor / intraocular pressure / cyclic AMP / Morphological change / aqueous humor dynamics / ocular penetration / melanin / cAMP分解酵素阻害剤 / 房水循環動態 / CAMP-poshodi esterase阻害剤 / 眼圧下降 / griseolic acid / 緑内障 |
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| Research Abstract |
1. REDUCTION IN INTRAOCULAR PRESSURE Topical application of 0.5% griseolic acid-ester (GA-ester) showed no significant ocular hypotensive response. But, a significant reduction in intraocular pressure (IOP) was observed at 2hr after topical application of 1% GA-ester. The ocular hypotensive response lasted 5 hrs after topical application of 2% GA-ester. IOP in rabbits was reduced in a dose-related manner following topical application of concentration of 0.5% to 2%.
2. cAMP ACCUMULATION IN IRIS-CILIARY BODY GA-ester at 100muM markedly increased the cAMP levels in rabbit iris-ciliary bodies. cAMP levels in GA-ester treated iris-ciliary body is four times higher than in IBMX treated one.
3. MORPHOLOGICAL CHANGES IN IRIS-CILIARY BODY Topical application of 2% GA-ester (50ul) caused nb morphological changes in ciliary process at 1 to 24h. No bemorrhage, no stromal edema nor invasion of inflammatory cells were observed. Even electron micrograph could not detect any morphological changes in the ciliary epithelium after instillation of 2% GA-ester.
4. CHANGES IN AQUEOUS HUMOR DYNAMICS The mean aqueous flow rate measured by fluorophotometry was significantly lower in GA- treated eyes than in control eyes. Topical application of Ga- ester did not alter tonographic outflow or uveoscleral outflow measured using fluorescein isothiocyanate labeled-dextran.
5. INTRAOCULAR PENETRATION OF GRISEOLIC ACID-ESTER 1 hour after topical application, 12.5%, 0.065%, O.010%, 0.017% and 0.010% of topically applied GA-ester was detected, respectively, in the conjunctive, cornes, aqueous humor, iris and ciliary body.
6. AFFINITY OF GRISEOLIC ACID -ESTER FOR SYNTHETIC MELANIN Timolol exhibited binding affinity to synthetic melanin. But, GA- ester did not bind to synthetic melanin.
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Report
(3 results)
Research Products
(22 results)
