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Histochemical studies on the distribution of calcium in the cells related to the formation of dental and periodontal hard tissues.

Research Project

Project/Area Number 63570847
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TAKANO Yoshiro  HOKKAIDO UNIVERSITY SCHOOL OF DENTISTRY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (90126425)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Tsuneyuki  HOKKAIDO UNIVERSITY SCHOOL OF DENTISTRY, RESEARCH ASSOCIATE, 歯学部, 助手 (80200822)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsENAMEL ORGAN / RAT / INCISOR / ENAMEL / CALCIUM / HISTOCHEMISTRY / RAPID FREEZING / FREEZE-SUBSTITUTION / 急速凍結凍結置換
Research Abstract

The distribution of calcium in the enamel organ, pulp and periodontal tissues was examined by means of histochemical, electron microscopic and X-ray microanalytical methods. All specimens were rapidly frozen/freeze-substituted and embedded in epoxy resin for the preservation of morphology and cell calcium. This method allowed a histochemical demonstration of cell calcium as diffuse or discrete red Ca-GBHA precipitates under the light microscope, and an electron microscopic examination of adjacent ultrathin sections.
Our study revealed intense calcium reactions in the cytoplasm of both secretory and maturation ameloblasts, and its absence in other enamel organ cells. Electron microscopy and X-ray microanalytical studies confirmed the presence of calcium-containing granules and crystalline figures in the tubulovesicular structures of secretory ameloblasts, implicating the contribution of such organelles in transcellular translocation of calcium. In the pulp, Ca-GBHA reactins were shown in differentiated odontoblasts but not in other pulp cells. In molar tooth germs at the stage of root formation, Ca-GBHA reactions appeared in cementoblasts and precementoblasts located at disrupted portions of unreactive epithelial root sheath. Osteoblasts and osteoclasts on the forming and absorbing surfaces of the alveolar bone also showed intense Ca-GBHA reactions in the cytoplasm.
In this project, we thus established a tissue preparation method whereby both cell morphology and unstable cell calcium is preserved. A histochemical calcium staining method was also established. With these methods, data suggesting the exclusive localization of high concentrations of calcium in cells directly related to formation or absorption of enamel, dentin or bone were presented. The elucidation of the physiological significance of present findings awaits further studies.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Takano Yoshihiro: "Cyclic changes in the properties of maturing enamel in the bovine incisor as revealed by glyoxal bis(2-hydroxyanil)staining." Archives of oral Biology. 33. 231-236 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano Yoshihiro: "Histochemical,ultrastructural and X-ray microanalytical studies of localization of calcium in the mucous lining of the rat duodenum." Histochemistry. 89. 429-436 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano Yoshihiro: "A histochemical demonstration of calcium in the maturation stage enamel organ of rat incisors." Archives of Histology and Cytology. 51. 241-248 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Wakisaka Satoshi: "Neurokinin A-like immunoreactivity in feline dental pulp:its distribution,origin and coexistence with substance P-like immunoreactirity" Cell and tissue Research. 251. 565-569 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo Saburou: "Effect of colchicine on the transport of precursor enamel protein in secretory ameloblasts studied by ^3H -proline radioautography in vitro." Anatomical Record. 221. 812-822 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo Saburou: "Budding of small vesicles from the rough-surfaced endoplasmic reticulum in secretory ameloblasts of rat molar tooth germs." Anatomical Record. 222. 317-322 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo Saburou: "Cytochemical study of the Golgi apparatus and related organelles of the secretory ameloblasts of rat molar tooth germs cultured with and without colchicine." Archives of oral Biology. 33. 433-441 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa Hiroyuki: "Occurrence of substance P-like immunoreactive nerve fibers in Krause apparatus of dog tongue." Acta Anatomica. 131. 215-217 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa Hiroyuki: "Coexistence of vasoactive intestinal polypeptide-and substance P-like immunoreactivities in the tongue of the guinea pig." Neuroscience Letters. 89. 283-285 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato Yukio: "Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes:Regulation by transforming growth factor β and serum factors." Proceedings of National Academy of Science,U.S.A.85. 9552-9556 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 大前博昭: "ニワトリ胚脛骨の器官培養系における骨膜未分化間葉系細胞の軟骨への分化" 歯科基礎医学会雑誌. 30. 646-651 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano Yoshiro: "Histochemical localization of calcium in the enamel organ of rat incisors in early-stage amelogenesis." Acta Anatomica. 134. 305-311 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 高野吉郎: "エナメル質石灰化の組織化学・カルシウム沈着のメカニズムをめぐって" 医学のあゆみ. 148. 215-218 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 高野吉郎: "カルシウム輸送上皮における細胞内カルシウムの局在について-成熟期エナメル芽細胞層と腸管吸収上皮の比較-" エナメル質比較発生学懇話会記録. 1. 5-8 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 脇田稔: "魚類エナメル質と象牙質の基質線維-ニザダイの歯について-" エナメル質比較発生学懇話会記録. 1. 33-36 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa Hiroyuki: "Substance P-like immunoreactive subepithelial nerve plexuses in the labial mucosa of the mouse." Acta Anatomica. 134. 283-285 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo Saburou: "Localization of two distinct acid phosphatases in secretory ameloblasts of rat molar tooth germs." Archives of oral Biology. 34. 599-608 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takigawa Masaharu: "Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes." Cancer Research. 49. 3996-4002 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano, Y.: "Cyclic changes in the properties of maturing enamel in the bovine incisor as revealed by glyoxal bis(2-hydroxyanil) staining." Archs Oral Biol., 33:231-236, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Wakisaka, S.: "Neurokinin A-like immunoreactivity in feline dental pulp: its distribution, origin and coexistence with substance P-like immunoreactivity." Cell Tissue Res., 251:565-569, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, H.: "Occurrence of substance P-like immunoreactive nerve fibers in Krause apparatus of dog tongue." Acta Anat., 131:215-217, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo, S.: "Effect of colchicine on the transport of precursor enamel protein in secretory ameloblasts studied by ^3H-proline radioautography in vitro." Anat. Rec., 221:812-822, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo, S.: "Budding of small vesicles from the rough-surfaced endoplasmic reticulum in secretory ameloblasts of rat molar tooth germs." Anat. Rec., 222:317-322, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo, S.: "Cytochemical study of the Golgi apparatus and related organelles of the secretory ameloblasts of rat molar tooth germs cultured with and without colchicine." Archs Oral Biol., 33:433-441, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano, Y.: "Histochemical, ultrastructural and X-ray microanalytical studies of localization of calcium in the mucous lining of the rat duodenum." Histochemistry, 89:429-436, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano, Y.: "A histochemical demonstration of calcium in the maturation stage enamel organ of rat incisors." Arch. Histol. Cytol., 51:241-248, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, H.: "Coexistence of vasoactive intestinal polypeptide- and substance P-like immunoreactivities in the tongue of the guinea pig." Neuroscience Letters, 89:283-285, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohmae, H.: "Chondrogenesis in the periosteum of chick embryonic tibia in vitro." Jpn. J. Oral Biol., 30:646-651, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, Y.: "Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes: Regulation by transforming growth factor beta and serum factors." Proc.Natl.Acad.Sci.USA, 85: 9552-9556, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kato, Y.: "Regulation of terminal differentiation of chondrocytes and mineralization of extracellular matrix of cartilage by growth factors and hormones." J. Jpn.Soc. Bone Mineral 7:16-20, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano, Y.: "Histochemical localization of calcium in the enamel organ of rat incisors in early-stage amelogenisis." Acta Anat., 134:305-311, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano, Y.: "Histochemical aspect of enamel mineralization with special reference to the mechanism of calcium acquisition in the enamel." Igaku-no-ayumi, 148:215-218, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takano, Y.: "Distribution of cell calcium in calcium transporting epithelia: a comparison between maturation ameloblasts and enterocytes." Archs Soc. Evol. Morphogenesis Dent. Enamel, 1:5-8, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Wakita, M.: "Matrix fibers in the enamel and dentin of the fish: on the teeth of Prionurus microlepidotus LCEPEDE." Archs Soc. Evol. Morphogenesis Dent. Enamel, 1:33-36, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ichikawa, H.: "Substance-P-like immunoreactive subepithelial nerve plexuses in the labial mucosa of the mouse." Acta Anat., 134:283-285, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuo, S.: "Localization of two distinct acid phosphatases in secretory ameloblasts of rat molar tooth germs." Archs Oral Biol., 34:599-608, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takigawa, M.: "Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes." Cancer Res., 49:3996-4002, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TAKANO YOSHIRO: "Histochemical localization of calcium in the enamel organ of rat incisors in early-stage amelogenesis." Acta Anatomica. 134. 305-311 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 高野吉郎: "エナメル質石灰化の組織化学・カルシウム沈着のメカニズムをめぐって" 医学のあゆみ. 148. 215-218 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 高野吉郎: "カルシウム輸送上皮における細胞内カルシウム局在について-成熟期エナメル芽細胞層と腸管吸収上皮の比較-" エナメル質比較発生学懇話会記録. 1. 5-8 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 脇田稔: "魚類エナメル質と象牙質の基質線維-ニザダイの歯について-" エナメル質比較発生学懇話会記録. 1. 33-36 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] ICHIKAWA HIROYUKI: "Substance P-like immunoreactive subepithelial nerve plexusesin the labial mucosa of the mouse." Acta Anatomica. 134. 283-285 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] MATSUO SABUROU: "Localization of two distinct acid phosphatases in secretory ameloblasts of rat molar tooth germs." Archives of oral Biology. 34. 599-608 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] TAKIGAWA MASAHARU: "Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes." Cancer Research. 49. 3996-4002 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 高野吉郎: 医学の歩み. 148. 215-218 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高野吉郎: エナメル質比較発生学懇話会記録. 1. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshiro Takano: Acta Anatomica. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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