| Project/Area Number |
63570848
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
NAGAI Noriyuki Okayama Univ.Dental Sch. Oral Pathology professor, 歯学部・口腔病理学講座, 教授 (90085770)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGI Takumi Okayama Univ.Dental Sch. Oral Pathology assistant, 歯学部・口腔病理学講座, 教職員 (50192878)
TUJI Takanori Okayama Univ.Dental Sch. Oral Pathology assistant prof, 歯学部・口腔病理学講座, 助手 (50201637)
ONO Toshirou Okayama Univ.Dental Sch. Oral Pathology assistant prof, 歯学部・口腔病理学講座, 助手 (50185641)
TAKESHITA Nobuyoshi Okayama Univ.Dental Sch. Oral Pathology associate prof, 歯学部・口腔病理学講座, 助教授 (00118275)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Reconstruction of jaw bone / Implant / Apatite / Macrophage / Osteoblastic cell / Mineralization / Microenviroment / 骨髄 / 初期石灰化 / 脂肪細胞 / オステオアンキロ-シス / マクロファージ / 表面荷電 / 顎骨骨髄 |
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| Research Abstract |
One of the most important theme in commencing aged society is a loss of occulsal function due to the missing of teeth. There are several treatments to be restored the loss of occulsal function in order to recover a cultural comfortable life, that is, reconstruction of jaw bone and dental implant. It is important to develop the systems to control and induce the osteoblastic differentiation of jaw bone cells before we select these methods and adaptable cases.
Year 1: We investigated relations between a surface structure of hydroxy-apatite and cell responses to it. We revealed the following facts; (1) There is a coupling mechanism between a cytodifferentiation of osteoblastic cells and an appearance of macrophages (Shirasuga et al). (2) Macrophages dissolve apatite in their risosomes (Nagai). (3) Osteo-ankylosis (osseocontact, osseointegrate) is necessary to stabilize the dental implants in jaw bone (Akagi).
Years 2: We examined the facts which influenced on them by in vitro studies in the multiple ways. Systems which we used were; apatite-osteogenic cells(HOS), apatite-periostium in culture bone marrow stromal cells of multi potential differentiation (Takesita,Ono,Tsuji). We revealed that apatite played a role of matrix material and related to mineralization at the interface. Microenviroment controlled the cytodifferntiation of osteoblastic cells.
From these facts, it was suggested that (1)microenviroment (PH,Ca^<2+> concentration) were related to osteogenic differentiation and mineralization of bone marrow stromal cells. (2)Apatite were also positively concerning with these dynamic aspects.
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