|Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
The present study investigated some lectin affinities of human, rat dental pulp, tooth germ of rat and transplanted pulp of rat incisor. Lectins used were ConA, WGA, RCA-1, UEA-1, DBA, SBA, MPA, LFA, HPA, PNA,and GS-1, and the avidin-biotin peroxydase complex method was employed. The following results were obtained:
1. On human dental pulp: (1) Lectin-binding in odontoblasts was intensely positive with ConA, WGA, RCA-1, MPA, and LFA. (2) The pulp cells were clearly positive with ConA, MPA, LFA, RCA-1, and SBA and especially LFA showed an intense reaction with the pulp cells. (3) Collagen fibers were clearly positive with MPA, LFA and RCA-1. (4) Bloodvessel walls were clearly positive with RCA-1, and nerve fibers were intensely positive with WGA. (5) It is clear that the lectin-binding pattern of odontoblasts differs from that of pulp cells. The data suggest that D-mannose, N-acetyl-D-glucosamine, D-galactose, and N-acetyl-galactosamine residues are localized in odontoblasts and sialic acid is localized in the pulp cells.
2. On dental pulp and tooth germ of rat inciser: (1) SBA- and PNA- binding showed different between pretreatment and non-treatment with neuraminidase. (2) Dentinal tubules and/or dentnal fibers were positive with only LFA. (3) Odontoblast s were clearly positive with SBA on pretreatment case with neuraminidase. (4) Ameloblasts were intensely positive with MPA, PNA and SBA. (5) It is clear that the lectin-binding pattern of odontoblasts differs from that or ameloblasts.
3. On the transplanted pulp of rat inciser; (1) The transplanted pulp was positive with ConA, WGA, and RCA-1, especially, WGA showed intensely positive. (2) Lectin-binding site was the front of calcification. (3) It was suggested that the front of calcification could be inferred to exhibit proteoglycan with a high concentration of N-acetyl-D-glucosamine and/or sialic acid.