| Research Abstract |
The purpose of this study was to investigate histologically and ultrastructurally the changes of epithelial cells in the tongue which was wounded twice and treated with Trp-P-2(3-amino-l-metliyl-5H -pyrido[4,3-b]indole).
1. Light Microscopy Hamsters were divided into 10 groups(Groups A-J). In all 10 groups, the tip(2mm) of the tongue was excised with scissors. In Groups A and F, the wound was painted trice weekly with DMSO(dimethyl sulf- oxide) for 8 weeks. In Groups B,C,D,G,H and I, the wound was painted trice weekly with a 0.5%solution of Trp-P-2 in DMSO for 8 weeks. In Groups E and J, the wound was not treated for 8 weeks. At the end of 8 weeks, in Groups A,B,C,D and E, the tip of the tongue was again excised, while in Groups F,G,H,I and J, the tip was not excised. In Groups A,C,F and H, the tongue was painted daily with DRSO until the animals were killed. In Groups B and G, the animals received no treatment until they were killed. In Groups D,E,l and J, the tongue was painted daily
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with Trp-P-2 until the animals were killed. Animals of all groups were killed aL 11 and 15 days after the second excision or after the end of 8 week. The tongue was examined histologically.
2. Electron Microscopy In Groups a,c and d, the animals received the same treatment as those of Groups A,C and D, respectively. The animals were killed at 15 days after the second excision. The tongue was examined ultra- structurally.
3. Light Microscopic Observation 1)In Groups A, at 11 and 15 days, the tongue showed only hyperkeratosis. 2)ln Groups B, at 11 and 15 days, the tongue snowed the changes of the mild epithelial dysplasia. 3)ln Groups C and D, at 11 and 15 days, the tongue showed the changes of the marked epithelial dysplasia. These changes were disorientation of cells, cellular and nuclear pleomorphisms, nuclear hyperchromatism, increased number of mitotic figures, abnormal keratinization and elongation of epithelial projection. 4)In Group E, at 11 and 15 days, the tongue showed the changes of the mild epithelial dysplasia. 5)In Groups F,G,H,I and J, the tongue showed only hyperkeratosis.
4. Electron Microscopic Observation 1)In Group a, the cells in the basal cell layer were round or ovoid in shape with large centrally-located nuclei. The basal lamitia just below the basal cells was almost intact, but on occasions absent or irregularly multilayered. The cells in the prickle cell layer were slightly flattened. The bundles of tonofilaments in the cytoplasm of these cells surrounded the nucleus or were oriented towards the desmosomes. The cells in the granular cell layer were moderately flattened and contained various amount of keratohyaline granules. The cells in the keratinized cell layer were very flattened and had no nucleus. 2)In Groups c and d, the cells in the basal cell layer were irregular in shape. The nucleus was enlarged and also appeared irregular in shape. The bundles of tonofilaments in the cytoplasm surrounded the nucleus or showed swirl-like arrangement. Occasionally, intranuclear tubular-like structures were observed within the nucleus. The basal lamina below the basal cells frequently absent or irregularly multilayered. The cells in the prickle cell layer were irregular in shape. In the cytoplasm of these cells, the bundles of tonofilaments surrounded the nucleus or scattered or localized or showed a swirl-like arrangement. Occasionally, virus-like particles and intranuclear tubular-like structures were observed within the nucleus. The cells in the granular cell layer were flattened. Sometimes, the nuclei of these cells contained virus-like particles. The cells in the keratinized cell layer usually lacked a nucleus, but on occasions had a nucleus with virus-like particles. Less
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