• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

TEMPORAL AND SPATIAL EXPRESSION PATTERN OF EPIDERMAL GROWTH FACTOR GENE DURING DEVELOPMENT OF MOUSE INCISOR

Research Project

Project/Area Number 63570870
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionOKAYAMA UNIVERSITY DENTAL SCHOOL

Principal Investigator

NOJI Sumihare  OKAYAMA UNIVERSITY DENTAL SCHOOL, ASSISTANT, 歯学部, 助手 (40156211)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Shigehiko  OKAYAMA UNIVERSITY DENTAL SCHOOL PROFESSOR, 歯学部, 教授 (50034161)
YAMAAI Tomoichiro  OKAYAMA UNIVERSITY DENTAL SCHOOL, ASSISTANT, 歯学部, 助手 (00158057)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsEpidermal growth factor gene / Retinoic acid receptor gene / mouse tooth / in situ hybridization / In situ hybridization / 上皮成長因子 / マウス顎下腺 / マウス切歯
Research Abstract

In the first year of the project term, we developed in situ hydridization method which is an indispensable method of this project. It took one year to obtain satisfactory results. We published the method in "Radioisotopes" in 1989. Then, we started to investigate expression patterns of the epidermal growth factor (EGF) gene during tooth development. unfortunately, we heard that Dr. Slavkin of South California University was going to publish the results what we intend to obtain. Thus, we decided to proceed our project. What we interested in next was what controls the expression of the EGF gene. Since it is likely that One of the candidates is retinoic acid, we investigated the expression pattern of its receptor (RAR). As results, we found that (1) RARalpha gene is expression in tissue of the mouse embryos at any time rather weakly. Thus, the gene may be a house-keeping gene. (2) RARbeta gene is expressed in the dorsal side of the spinal cord and face of the embryo (10-13 day old) including tooth. Furthermore, the expression is closely related to development of the sensory organs such as nose, ear, eye, and also to programmed cell death. (3) RARgamma gene is expressed in the cells which become cartilages, bones, or teeth. Furthermore. the gene expression is closely related to the development and differentiation of skin.
From these results, we concluded that RA plays important roles in development of tooth including incisors. Since the expression pattern of the RAR genes resembles to that of the EGF genes, the expression of the EGF genes may be controlled by the RAR-RA complexes.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 野地澄晴、山合友一朗、小山英樹、谷口茂彦: "リボプロ-ブを用いた改良in situハイブリダイゼ-ション法による上皮成長因子および神経成長因子遺伝子のマウス顎下腺における発現部位" Radioi sotopes. 38. 366-372 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Noji,T.Yamaai,E.Koyama,T.Nohno and S.Taniguchi: "Spatial and temporal expression pattern of retinoicaeid receptor genesduring mouse bone development." FEBS Lett.257. 93-96 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Noji,T.Yamaai,E.Koyama,T.Nohno,W.Fujimoto,J,Arata S.Taniguchi: "Explession of retinoicacid receptor genes in keratinizing front of skin" FEBS Lett.259. 86-90 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 四童子好広、野地澄晴: "レイノチン酸の生理機能" 代謝. 26. 327-340 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Osumi-Yamashita,S.Noji et al.: "Expression of retinoic acid receptor genes in the neural crest derived cells during mouse facial development" FEBS Lett.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sumihare Noji, Tomoichiro Yamaai, Eiki Koyama, Shigehiko Taniguchi: "Expression of EGF and NGF genes in mouse submandibular gland, as revealed by modified in situ hybridization with riboprobes." Radioisotopes. 38,. 366-372. (1989,)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sumihare Noji, Tomoichiro Yamaai, Eiki Koyama, Tsutomu Nohno, Shigehiko Taniguchi: "Spatial and temporal expression pattern of retinoic acid receptor genes during mouse bone development." FEBS Lett.,. 257,. 93-96. (1989,)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sumihare Noji, Tomoichiro Yamaai, Eiki Koyama, Tsutomu Nohno, Wataru Fujimoto. Jiro Arata, Shigehiko Taniguchi: "Expression of retinoic acid receptor genes in keratinizing front of skin" FEBS Lett.,. 259,. 86-90. (1989,)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshihiro Shidoji and Sumihare Noji: "Physiological function of retinoic acid" Taisha. 26,. 327-340 (1989,)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriko Osumi-Yamashita, Sumihare Noji, et al.: "Expression of retinoic acid receptor genes in the neural crest derived cells during mouse facial development" FEBS Lett. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 野地澄晴、山合友一朗、小山英樹、谷口茂彦: "リボプロ-ブを用いた改良in situハイブリダイゼ-ション法による上皮成長因子および神経成長因子遺伝子のマウス顎下腺における発現部位" Radioi sotopes. 38. 366-372 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Noji,T.Yamaai,E.Koyama,T.Nohno and S.Taniguchi: "Spatial and temporal eppression pattern of retinoic aeid receptor genes during mouse bone derelopment." FEBS Lett.257. 93-96 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Noji,T.Yamaai,E.Koyama,T.Nohno,W.Fujimoto,J.Arata,S.Taniguchi: "Expression of retinoic acid receptor genes in Keratinizing front of skin" FEBS Lett.259. 86-90 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 四童子好広、野地澄晴: "レチノイン酸の生理機能" 代謝. 26. 327-340 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Osumi-Yamashita,S.Noji et al.: "Expression of retinoic acid receptor genes in the neural crest derived Cells during mouse facial development" FEBS Lett.

    • Related Report
      1989 Annual Research Report
  • [Publications] 野地澄晴: RADIOISOTOPES.

    • Related Report
      1988 Annual Research Report
  • [Publications] 野地澄晴: J.Histochem.Cytochem.

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1988-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi