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Attachment and invasion of periodontopathic bacteria tosubgingival site

Research Project

Project/Area Number 63570878
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

NAITO Yuko  Tokyo Dental College, Dep. Microbiology, Assistant Professor, 歯学部・微生物, 講師 (00147258)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Kazuyuki  Tokyo Dental College, Dep. Microbiology, Research Assistant, 歯学部・微生物, 助手 (00212910)
KATO Tetsuo  Tokyo Dental College, Dep. Microbiology, Research Assistant, 歯学部・微生物, 助手 (00159253)
OHTA Kousei  Tokyo Dental College, Dep. Microbiology, Associated Professor, 歯学部・微生物, 助教授 (30138680)
OKUDA Katsuji  Tokyo Dental College, Dep. Microbiology, Professor, 歯学部・微生物, 教授 (40085741)
小野 美千代  東京歯科大学, 歯学部・微生物学教室, 助手 (60167327)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBACTEROIDES GINGIVALIS / COLLAFGEN / ADULT PERIODONTITIS / ATTACHMENT FACTOR / hydroxyapatite / fibronectin
Research Abstract

Bacteroides gingivalis has been associated with adult periodontitis in humans. The organism have been shown to attach to experimental saliva pellicles formed on mineral surfaces similar to those to teeth. Recently, adsorbed acidic saliva proline-rich proteins (PRP) have been found to promote the attachment of B.gingivalis cells to hydroxyapatite surfaces. These salivary proteins have some peptide sequences in common with various types of collagen. Since collagen is a major component of the periodontium, and is destroyed during the course of periodontal disease, we investigated the ability of B. gingivalis cells to bind to collagenous substrata.
1.Treating HA with either type 1 or type 4 human collagen(C-HA) greatly promoted attachment of B.gingivalis cells.Heating B. gingivalis cells destroyed their ability to attach to type 1 C-HA.The individual strains of B. gingivalis differ in their ability to adhere to C-HA. 2.Human IgG, fibronectin, fibrinogen, human serum and some peptide i.e. gly-gln, gly-asp, pro-gln-gly inhibited attachment to C-HA. 3.An overall correlation was noted between the hydrophobicity and their numbers which attached to C-HA. 4.Pretreatment of bovine bone collagen with fibronection and laminin decreased the number of B. gingivalis 33277 cells and the other cells which attached C-HA, however W83 cells were increased to attach to the C-HA with fibronectin. 5.The bacterial strain which have a number of fimbraie on their surface, greatly attached to C-HA, while some B. ginjivalis strains having a few fimbrae were not binding to C-HA.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Naito,Y.,R.J.Gibbons: "Attachment of Bacteroides gingivalis to collagenous substrata" J.Dent.Res.67. 1075-1080 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naito,Y.,N.Ito,K.Okuda and I.Takazoe: "The heterogeneity of outer membrane of selected aral black-pigmented Bacteroides species." Bull.Tokyo dent.Coll.29. 111-118 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 内藤祐子: "Bacteroides gingivalis の付着性についてーHydrooxyapatite beadsを用いてー" 日細菌誌. 45. 465 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Osawa: "Inhibitory effect of Bacteroides gingivalis collagenolytic activity by an aqucous extract of Cacao Bean Husk" Bull.Tokyo dent.Coll.31. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石原和幸: "歯周炎病原菌由来の酵素活性を指標とした迅速診断法 Sk-013の特性と酵素特異性の検討" 日歯周誌. 32. 225-231 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 清田築: "歯周炎病原菌由来の酵素活性を指標とした迅速診断法(SKー013) 細菌叢におけるB.gingivalis,B.forsythus,T.denticolaとの関連性" 日歯周誌. 32. 232-240 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naito, Y., and R.J.Gibbons: "Attachment of Bacteroides gingivalis to collagenous substrata" J.Dent.Res. 67:1075-1080, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naito, Y., N.Ito, K.Okuda, and I.Takazoe: "The heterogeneity of outer membrane of selected oral black-pigmented Bacteroides species." Bull Tokyo dent Coll. 29:111-118, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naito, Y., K.Okuda, and I.Takazoe: "The role of attachment of Bacteroides gingivalis" Jpn. J. Bacteriol. 45:465, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Osawa, K., T.Matumoto, Y.Naito, K.Okuda, and I.Takazoe: "Inhibitory effect of Bacteroides gingivalis collagenolytic activity by an aqueous extract of Cacao Bean Husk" Bull Tokyo dent Coll 31, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishihara, K., Y.Naito, T.Kato, I.Takazoe, and K.Okuda, et al.: "A rapid diagnosis(Sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria(1)" Jpn.J.Perio. 32:224-240, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Seida, K., A.Saito, R.Yamada, K.Ishihara, Y.Naito, K.Okuda, et al.: "A rapid diagnosis(Sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria(2)" Jpn.J.Perio. 32:240-253, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 内藤祐子: "Bacteroides gingivalisの付着性について-Hydrooxyapatite beadsを用いて-" 日細菌誌. 45. 465 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Osawa: "Inhibitory effect of Bacteroides gingivalis collagenolytic activity by an aqueous extract of Cacao Bean Husk" Bull Tokyo dent Coll. 31. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石原和幸: "歯周炎病原菌由来の酵素活性を指標とした迅速診断法 Sk-013の特性と酵素特異性の検討" 日歯周誌. 32. 225-231 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 清田築: "歯周炎病原菌由来の酵素活性を指標とした迅速診断法(Sk-013)細菌叢におけるB.gingivalis,B.forsythus,T.denticolaとの関連性" 日歯周誌. 32. 232-240 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Naito,Y.;R.J.Gibbons: J.Dent.Res.67. 1075-1080 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Naito,Y.;N.Ito;K.Okuda;I.Takazoe: Bull.Tokyo dent.Coll.29. 111-118 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 内藤祐子,R.J.Gibbons: 日細菌誌. 43. 395 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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