| Project/Area Number |
63570878
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
NAITO Yuko Tokyo Dental College, Dep. Microbiology, Assistant Professor, 歯学部・微生物, 講師 (00147258)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Kazuyuki Tokyo Dental College, Dep. Microbiology, Research Assistant, 歯学部・微生物, 助手 (00212910)
KATO Tetsuo Tokyo Dental College, Dep. Microbiology, Research Assistant, 歯学部・微生物, 助手 (00159253)
OHTA Kousei Tokyo Dental College, Dep. Microbiology, Associated Professor, 歯学部・微生物, 助教授 (30138680)
OKUDA Katsuji Tokyo Dental College, Dep. Microbiology, Professor, 歯学部・微生物, 教授 (40085741)
小野 美千代 東京歯科大学, 歯学部・微生物学教室, 助手 (60167327)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | BACTEROIDES GINGIVALIS / COLLAFGEN / ADULT PERIODONTITIS / ATTACHMENT FACTOR / hydroxyapatite / fibronectin |
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| Research Abstract |
Bacteroides gingivalis has been associated with adult periodontitis in humans. The organism have been shown to attach to experimental saliva pellicles formed on mineral surfaces similar to those to teeth. Recently, adsorbed acidic saliva proline-rich proteins (PRP) have been found to promote the attachment of B.gingivalis cells to hydroxyapatite surfaces. These salivary proteins have some peptide sequences in common with various types of collagen. Since collagen is a major component of the periodontium, and is destroyed during the course of periodontal disease, we investigated the ability of B. gingivalis cells to bind to collagenous substrata.
1.Treating HA with either type 1 or type 4 human collagen(C-HA) greatly promoted attachment of B.gingivalis cells.Heating B. gingivalis cells destroyed their ability to attach to type 1 C-HA.The individual strains of B. gingivalis differ in their ability to adhere to C-HA. 2.Human IgG, fibronectin, fibrinogen, human serum and some peptide i.e. gly-gln, gly-asp, pro-gln-gly inhibited attachment to C-HA. 3.An overall correlation was noted between the hydrophobicity and their numbers which attached to C-HA. 4.Pretreatment of bovine bone collagen with fibronection and laminin decreased the number of B. gingivalis 33277 cells and the other cells which attached C-HA, however W83 cells were increased to attach to the C-HA with fibronectin. 5.The bacterial strain which have a number of fimbraie on their surface, greatly attached to C-HA, while some B. ginjivalis strains having a few fimbrae were not binding to C-HA.
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