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Cell Biological Approach to the Novel Diagnosis and Treatment of Oral Cancer

Research Project

Project/Area Number 63570933
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionTokyo Medical and Dental University

Principal Investigator

HORIKOSHI Masaru  Tokyo Med. Staff and Dent. Univ., 歯学部, 助手 (00014283)

Co-Investigator(Kenkyū-buntansha) RIKIMARU Koichi  Tokyo Med. Staff and Dent. Univ., 歯学部, 助手 (40220800)
KUSAMA Mikio  Tokyo Med. Staff and Dent. Univ., 歯学部, 助手 (60124690)
IWASA Toshiaki  Tokyo Med. Staff and Dent. Univ., 歯学部, 助手 (10107302)
Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsOral Cancer / Sguamous cell carcinoma / human Epidermal growth factor / invasion model / Interleukin-1 / cytokine / Bone resorption / Parathyroid hormone related protein / インタ-ロイキン1 / 完全合成無蛋白培地 / IL-1 / DNA合成促進物質 / 無血清培養 / 細胞成長因子
Research Abstract

Squamous cell carcinoma (SCC)represents about 90% of total oral cancers. It was reported that the growth of SCC cells was inhibited by epidermal growth factor (EGF) at the dose which is stimulatory for the growth of other type of cancer cells. In this project it was revealed that the third disulfide loop from N-terminal of human EGF was very important for their growth suppressive function. To investigate on the possibility of the usage of EGF for the treatment of SCC, further studies, such as in vivo study using nude mice, should be carried out.
An in vitro model was developed to study the mechanisms of invasion in oral cancer, where SCC cells were cultured on the particular matrix consisting of collagen gel and human living fibroblasts. This model demonstrated that fibroblasts was implicated in the invasion in SCC cells in vitro, and each cell line presented the similar pattern of invasion as observed in original tumor specimen.
Furthermore, we developed a novel protein-free medium, designated PF86-1, for the culture of SCC cells. Since the protein-free culture system using PF86-1 seems to have significant advantage for the study on the tumor products, which may be implicated in the clinical behavior of tumor cells, we attempted to identify the bioactive substances secreted by SCC cells into the culture medium. In the study, it was shown that although SCC cells produced IL-1alpha and IL-1beta, only IL-1alpha was secreted into culture medium and IL-1beta was retained in the cytoplasm. Further study revealed that IL-1alpha might be an important factor causing hypercalcemia in SCC. Patients via its bone resorbing activity, as suggested by the result that IL-1alpha produced by SCC cells promoted the release of ^<45>Ca from the pre-labeled mouse calvariae in vitro.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Matsumoto K: "A study of an in vitro model for invasion of oral squamous cell carcinoma" J.Oral Pathology and Medicine. 18. 498-501 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tadokoro K: "Activation of oncogenes in human oral cancer cell:anovel cod'on 13 mutation of c-H-ras-1 and concurrent amplification of c-erbB-1 and c-myc." Oncogene. 4. 499-505 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 立川敬子: "口腔扁平上皮癌細胞の産生する骨吸収促進因子に関する研究" 日本口腔外科学会誌. 36. 1-15 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsumoto K: "A Study of an in vitro model for invasion of oral squamous cell carcinoma" J. Oral Pathology and Medicine vol.18 p498-501 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tadokoro K: "Activation of oncogenes in human oral cancer cells: a novel cod on 13 mutation of c-H-ras-1 and concurrent amplification of c-erbB-1 and c-myc." Oncogene vol.4 p499-505 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Noriko. T: "Bone resorbing factors produced by oral squamous cell carcinoma" Jpn. J. Oral Maxillofac. Surg. vol.36 No.4 1990 p1-15.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsumoto K: "A study of an in vitro model for invasion of oral squamous cell carcinoma" J.Oral.Pathology and Medicine. 18. 498-501 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadokoro K: "Activation of oncogenes in human oral cancer cells:in novel codon13 mutation of c-H-ras-1 and condurrent amplification of c-erbB-1 and c-myc" Oncogene. 4. 499505 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 立川敬子: "口腔扁平上皮癌細胞の産生する骨吸収促進因子に関する研究" 日本口腔外科学会誌. 36. 1-15 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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