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Systematic Search for New Pharmacologically Active Metabolites of Ascomycetes and Basidiomycetes

Research Project

Project/Area Number 63570981
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Chemical pharmacy
Research InstitutionChiba University

Principal Investigator

FUJIMOTO Haruhiro  Chiba University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (50089603)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsAscomycetes / Basidiomycetes / Pharmacologically active metabolites / Systematic search / Lethal toxicity / Monoamine oxidase inhibitory activity / Anti-acetylcholine effect / Cellular immunity / 系統的探索 / 遅延型過敏反応
Research Abstract

1. Toxicity of the crude extracts of more than fifty strains in Ascomycetes belonging to the genera, Talaromyces, Eupenicillium, Neosartorya, Hamigera and Eurotium was investigated by monitoring whether any extraordinariness occurs or no in mice by administration of the extract. On this bioassay, Eupenicillium hirayamae and Neosartorya fischeri were newly discovered to be toxic to mice. The toxic principles of E. hirayamae and N. fischeri were disclosed to be a known azaphilone compound and a new 1-hydroxy-2-pyridone compound, respectively. On a systematic monitoring monoamine oxidase inhibitory (HAOI) activity, the crude extract of Talaromyces luteus afforded MAOI effect. The MAOI principles of the fungus, tentatively named TL-1 and -2, were clarified to be new azaphilme compounds being isomeric each other. Investigation of any possibility to apply TL-1 and -2 to Parkinson disease is now remained to be our next project.
2. Toxicity of the crude extracts of some species in Basidiomycete … More s collected in Chiba, Yamanashi and Nagano was investigated in the same way as for the assay of Ascomycetes. On this bioassay, Hebeloma spoliatum was newly discovered to be lethally toxic to mice. Among three toxic principles, tentatively named HS-A, -B and -C, of the fungus, HS-A was identical with a known cytotoxic triterpene compound already isolated from the closely related species and other two were new compounds having the related structures to that of HS-A. The main lethal toxicity of these compounds may be originated from any disorder occurred in the central nervous system, but these compounds seem to be also effective to the digestive organs. They showed an anti-acetylcholine effect on a Magnus assay with a mouse small intestine preparation. Their biological activity will be further studied in near future.
3. Some methods to search systematically for imunologically active metabolites in Asco- and Basidiomycetes were studied. It was revealed to be a good method to search for any fungal metabolites being effective to the cellular immunity system to adopt firstly lymphs, cyte transformation test in vitro and then delayed type hypersensitization test in vivo. Less

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 藤本治宏、持斎康弘、堀江義一、山崎幹夫: "Talaromyces panasenkoiからの毒性代謝産物としてのspiculisporic acidの分離について" マイコトキシン. 27. 15-19 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto,T.Matsudo,A.Yamaguchi,M.Yamazaki: "Two New Fungal Azaphilones from Talaromyces luteus,with Monoamine Oxidase Inhibitory Effect" Heterocycles. 30. 607-616 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto,K.Maeda,M.Yamazaki: "New Toxic Metabolites from a Mushroom,Hebeloma vinosophyllum III.Isolation and Structures of Hebevinosides XII,XIII,and XIV" Chem.Pharm.Bull.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Gujimoto,Y.Takano,M.Yamazaki: "Isolation,Structures,and Some Pharmacological Properties of Toxic Metabolites from a Mushroom,Hebeloma spoliatum" Chem.Pharm.Bull.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 藤本治宏: "きのこ生理活性物質(総説)" 遺伝. 42(9). 25-29 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto, Y.Jisai, Y.Horie and M.Yamazaki: "On Isolation of Spiculisporic Acid, a Toxic Metabolite from Talaromyces panasenkoi" Proc. Jpn. Assoc. Mycotoxicol., No.27, 15-19, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto, T.Matsudo, A.Yamaguchi and M.Yamazaki: "Two New Fungal Azaphilones from Talaromyces luteus, with Monoamine Oxidase Inhibitory Effect" Heterocycles, 30, 607-616, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto, K.Maeda and M.Yamazaki: "New Toxic Metabolites from a Mushroom, Hebeloma vinosophyllum III. Isolation and Structures of Hebevinosides XII, XIII and XIV" Chem. Pharm. Bull.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto, Y.Takano, M.Yamazaki: "Isolation, Structures, and Some Pharmacological Properties of Toxic Metabolites from a Mushroom, Hebeloma spoliatum" Chem. Pharm. Bull.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H.Fujimoto,T.Matsudo,A.Yamaguchi,M.Yamazaki: "Two New Fungal Azaphilones from Talaromyces luteus,with Monoamine Oxidase Inhibitory Effect" Heterocycles. 30. 607-616 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Fujimoto,K.Maeda,M.Yamazaki: "New Toxic Metabolites from a Mushroom,Hebeloma vinosophyllum III.Isolation and Structures of Hebevinosides XII,XIII,and XIV" Chem.Pharm.Bull.

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Fujimoto,Y.Takano,M.Yamazaki: "Isolation,Structures,and Some Pharmacological Properties of Toxic Metabolites from a Mushroom,Hebeloma spoliatum" Chem.Pharm.Bull.

    • Related Report
      1989 Annual Research Report
  • [Publications] 藤本治宏、持斎康弘、堀江義一、山崎幹夫: マイコトキシン. 27. 15-19 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Fujimoto.;A.Yamaguchi,;T.Matsudo,;M.Yamazaki,: Chem.Pharm.Bull.37. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Fujimoto.;K,Maeda,;M,Yamazaki,: Chem.Pharm.Bull.37. (1989)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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