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Study on the cascade of gene expression induced by tumor promoters.

Research Project

Project/Area Number 63571037
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionInstitute of Medical Science, University of Tokyo

Principal Investigator

NOSE Kiyoshi  Inst.Med. Sci., Univ. Tokyo, Associate Professor, 医科学研究所, 助教授 (70012747)

Project Period (FY) 1988 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordstumor promoter / cDNA / osteoblastic cells / osteopontin / cDNA / Cーキナ-ゼ / 細胞増殖 / 発癌プロモーター / 遺伝子クローニング / 転写制御
Research Abstract

cDNA clones which were induced late by a tumor promoting phorbol ester TPA were isolated from a cDNA library of mouse osteoblastic cells. Eight clones were isolated, and from sequencing analysis three of them were metallothionein, one was osteopontin and the others were novel genes. Among them, OTS-8 gene was of particular interest, since its expression was transient and became maximum 4 hr after. TPA-treatment. The open reading frame of OTS-8 gene indicated to code a 26 kDa novel protein. The expression of the above genes were inhibited by an inhibitor of protein kinase C. The expression of osteopontin (OTS-5) and OTS-8 was sensitive to an inhibitor of protein synthesis. Although transcriptional enhancer of OTS-5 gene is reported to contain AP-1 site, its expression was not altered by expression of c-fos.

Report

(3 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Nose,K.: "Abolishment of c-fos inducibility in ras-transformed mouse osteoblastic cell line." Mol.Carcinogenesis. 2. 208-216 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Saito,H.: "Decrease in Cu Zn-superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells." FEBS Lett.249. 253-256 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashiba,H.: "Induction of metallothionein mRNA by tumor promoters in mouse skin and its constitutive expression in papillomas." Mol.Carcinogenesis. 2. 95-100 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shibanuma,M.: "Stimulation by hydrogen peroxide of DNA synthesis,competence family gene expression and phosphorylation of a specific protein in quiescent Balb/3T3 cells." Oncogene. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nose, K., Itami, M., Satake, M., Yoshiaki, Ito and Kuroki, T.: "Abolishment of c-fos inducibility in rastransformed mouse osteoblastic cell line." Mol. Carcinogenesis, 2, 208-216.1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Saito, H., Kuroki, T. and Nose, K.: "Decrease in Cu Zn-superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells." FEBS Letters, 249, 253-256, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashiba, H., Hosoi, J., Karasawa, M., Yamada, S., Nose, K. and Kuroki, T.: "Induction of metallothionein mRNA by tumor promoters in mouse skin and its constitutive expression in papillomas." Mol. Carcinogenesis, 2, 95-100, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shibanuma, M., Kuroki, T. and Nose, K.: "Stimulation by hydrogen peroxide of DNA synthesis, competence family gene expression and phosphorylation of a specific protein in quiescent Balb/3T3 cells." Oncogene 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nose,k.: "Abolishment of c-fos inducibility in ras-transformed mouse osteoblastic cell line." Mol.Carcinogenesis. 2. 208-216 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Saito,H.: "Decrease in Cu Zn-superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells." FEBS Lett.249. 253-256 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hashiba,H.: "Induction of metallothionein mRNA by tumor promoters in mouse skin and its constitutive expression in papillomas." Mol.Carcinogenesis. 2. 95-100 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shibanuma,M.: "Stimulation by hydrogen peroxide of DNA synthesis,competence family gene expression and phosphorylation of a specific protein in quiescent Balb/3T3 cells." Oncogene. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shibanuma,M.: J.Cellular Physiology. 136. 379-383 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shibanuma,M.: Oncogene. 3. 17-21 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Huang.M: Molecular Carcinogenesis. 1. 109-115 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 野瀬清: 細胞工学. 7. 678-685 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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