| Project/Area Number |
63571053
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
YAMAGUCHI Masayoshi University of Shizuoka School of Pharmaceutical Sciences, 薬学部, 講師 (70046308)
|
|---|
| Project Period (FY) |
1988 – 1989
|
| Project Status |
Completed (Fiscal Year 1989)
|
| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1988: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Regucalcin / Ca^<2+>-Binding Protein / Liver Cell Function / Ca^<2+>-Dependent Enzyme / Protein Kinase / Ca^<2+> Transport / カウシウム結合蛋白質 / Ca^<2+>-ATPase / グルコース-6-ホスファターゼ |
|---|
| Research Abstract |
1) ^<125>I-Regucalcin binds to isolated liver plasma membranes, and activated (Ca^<2+>-Mg^<2+>)-adenosine triphosphatase which functions as Ca^<2+> PUMP.
2) Ca^<2+> increased glucose-6-phosphatase activity in rat liver microsomes. The Ca^<2+> effect was reversed by regucalcin. Such effect was not seen in other metals.
3) Regucalcin activated Ca^<2+>-adenosine triphosphatase in rat liver microsomes. This suggested the possibility that regucalcin regulates Ca^<2+> transport system.
4) Regucalcin stimulated Ca^<2+> release from rat liver microsomes.
5) Regucalcin inhibited protein kinase C activity in rat liver cytosol.
6) Regucalcin inhibited Ca^<2+>/calmodulin-dependent protein kinase activity in rat liver cytosol.
From these findings, it has been demonstrated that a novel Ca^<2+>-binding protein regucalcin regulates liver cell functions related to Ca^<2+>.
|
